ELANE
Purified Mouse Monoclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application ![]()
| WB, IHC, ICC, E |
---|---|
Primary Accession | P08246 |
Reactivity | Human, Rat |
Host | Mouse |
Clonality | Monoclonal |
Clone Names | 6B6G6 |
Isotype | Mouse IgG1 |
Calculated MW | 28.5kDa |
Immunogen | Purified recombinant fragment of human ELANE (AA: 140-267) expressed in E. Coli. |
Formulation | Purified antibody in PBS with 0.05% sodium azide |
Gene ID | 1991 |
---|---|
Other Names | GE; NE; HLE; HNE; ELA2; SCN1; PMN-E |
Dilution | E~~ 1/10000 WB~~ 1/500 - 1/2000 |
Storage | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Precautions | ELANE is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | ELANE |
---|---|
Synonyms | ELA2 |
Function | Serine protease that modifies the functions of natural killer cells, monocytes and granulocytes. Inhibits C5a-dependent neutrophil enzyme release and chemotaxis (PubMed:15140022). Promotes cleavage of GSDMB, thereby inhibiting pyroptosis (PubMed:36899106). Promotes blood coagulation (PubMed:20676107). Through the activation of the platelet fibrinogen receptor integrin alpha-IIb/beta-3, potentiates platelet aggregation induced by a threshold concentration of cathepsin G (CTSG) (PubMed:25211214, PubMed:9111081). Cleaves and thus inactivates tissue factor pathway inhibitor (TFPI) (PubMed:20676107, PubMed:25211214). Capable of killing E.coli but not S.aureus in vitro; digests outer membrane protein A (ompA) in E.coli and K.pneumoniae (PubMed:10947984). |
Cellular Location | Cytoplasmic vesicle, phagosome. Note=Localized in phagolysosomes following ingestion of E.coli by neutrophils |
Tissue Location | Bone marrow cells. Neutrophil (PubMed:10947984). |

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Provided below are standard protocols that you may find useful for product applications.
References
1.Pediatr Blood Cancer. 2015 Dec;62(12):2229-31. 2.Lung. 2014 Aug;192(4):595-9.

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