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ADORA2A

Purified Mouse Monoclonal Antibody

     
  • E - ADORA2A AO2628a
    Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
    detail
  • WB - ADORA2A AO2628a
    Figure 2:Western blot analysis using ADORA2A mAb against human ADORA2A (AA: 274-412) recombinant protein. (Expected MW is 37 kDa)
    detail
  • WB - ADORA2A AO2628a
    Figure 3:Western blot analysis using ADORA2A mAb against HEK293 (1) and ADORA2A (AA: 274-412)-hIgGFc transfected HEK293 (2) cell lysate.
    detail
  •  - ADORA2A AO2628a
    Figure 5:Flow cytometric analysis of Hela cells using ADORA2A mouse mAb (green) and negative control (red).
    detail
  • ICC - ADORA2A AO2628a
    Figure 4:Immunofluorescence analysis of Hela cells using ADORA2A mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
    detail
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IHC, ICC, E
Primary Accession P29274
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 6A6G11
Isotype Mouse IgG2a
Calculated MW 44.7kDa
Immunogen Purified recombinant fragment of human ADORA2A (AA: 274-412) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide
Additional Information
Gene ID 135
Other Names A2aR; RDC8; ADORA2
Dilution E~~ 1/10000
WB~~ 1/500 - 1/2000
ICC~~ 1/100 - 1/500
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsADORA2A is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ADORA2A
Synonyms ADORA2
Function Receptor for adenosine (By similarity). The activity of this receptor is mediated by G proteins which activate adenylyl cyclase (By similarity).
Cellular Location Cell membrane {ECO:0000250|UniProtKB:P30543}; Multi-pass membrane protein {ECO:0000250|UniProtKB:P30543} Note=Colocalizes with GAS2L2 at neuronal processes {ECO:0000250|UniProtKB:P30543}
Research Areas
Citations (0)
citation

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References

1.Mol Neurobiol. 2015 Aug;52(1):664-78.2.J Psychiatr Res. 2014 Apr;51:49-59.

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$ 385.00
Cat# AO2628a
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