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ALDOA

Purified Mouse Monoclonal Antibody

     
  • E - ALDOA AO2616a
    Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
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  • WB - ALDOA AO2616a
    Figure 2:Western blot analysis using ALDOA mAb against human ALDOA (AA: 9-145) recombinant protein. (Expected MW is 40.7 kDa)
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  • WB - ALDOA AO2616a
    Figure 3:Western blot analysis using ALDOA mAb against HEK293 (1) and ALDOA (AA: 9-145)-hIgGFc transfected HEK293 (2) cell lysate.
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  • WB - ALDOA AO2616a
    Figure 4:Western blot analysis using ALDOA mouse mAb against MCF-7 (1), Hela (2), and NIH/3T3 (3) cell lysate.
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  •  - ALDOA AO2616a
    Figure 5:Flow cytometric analysis of K562 cells using ALDOA mouse mAb (green) and negative control (red).
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  • IHC - ALDOA AO2616a
    Figure 6:Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using ALDOA mouse mAb with DAB staining.
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  • IHC - ALDOA AO2616a
    Figure 7:Immunohistochemical analysis of paraffin-embedded breast cancer tissues using ALDOA mouse mAb with DAB staining.
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  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IHC, ICC, E
Primary Accession P04075
Reactivity Human, Mouse
Host Mouse
Clonality Monoclonal
Clone Names 1C5B2
Isotype Mouse IgG2a
Calculated MW 39.4kDa
Immunogen Purified recombinant fragment of human ALDOA (AA: 9-145) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide
Additional Information
Gene ID 226
Other Names ALDA; GSD12; HEL-S-87p
Dilution E~~ 1/10000
WB~~ 1/500 - 1/2000
IHC~~ 1/200 - 1/1000
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsALDOA is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ALDOA (HGNC:414)
Synonyms ALDA
Function Catalyzes the reversible conversion of beta-D-fructose 1,6- bisphosphate (FBP) into two triose phosphate and plays a key role in glycolysis and gluconeogenesis (PubMed:14766013). In addition, may also function as scaffolding protein (By similarity).
Cellular Location Cytoplasm, myofibril, sarcomere, I band {ECO:0000250|UniProtKB:P00883}. Cytoplasm, myofibril, sarcomere, M line {ECO:0000250|UniProtKB:P00883}. Note=In skeletal muscle, accumulates around the M line and within the I band, colocalizing with FBP2 on both sides of the Z line in the absence of Ca(2+) {ECO:0000250|UniProtKB:P00883}
Research Areas
Citations (0)
citation

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References

1.Cancer Lett. 2016 Apr 28;374(1):127-35. 2.Oncol Rep. 2014 Nov;32(5):2031-7.

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$ 385.00
Cat# AO2616a
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