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CHRNA4

Purified Mouse Monoclonal Antibody

     
  • E - CHRNA4 AO2581a
    Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
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  • WB - CHRNA4 AO2581a
    Figure 2:Western blot analysis using CHRNA4 mAb against human CHRNA4 (AA: 29-242) recombinant protein. (Expected MW is 52.5 kDa)
    detail
  • WB - CHRNA4 AO2581a
    Figure 3:Western blot analysis using CHRNA4 mAb against HEK293 (1) and CHRNA4 (AA: 29-242)-hIgGFc transfected HEK293 (2) cell lysate.
    detail
  • WB - CHRNA4 AO2581a
    Figure 4:Western blot analysis using CHRNA4 mouse mAb against SH-SY5Y (1) cell lysate.
    detail
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IHC, ICC, E
Primary Accession P43681
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 7B4H1
Isotype Mouse IgG1
Calculated MW 70kDa
Immunogen Purified recombinant fragment of human CHRNA4 (AA: extra 29-242) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide
Additional Information
Gene ID 1137
Other Names EBN; BFNC; EBN1; NACHR; NACRA4; NACHRA4
Dilution E~~ 1/10000
WB~~ 1/500 - 1/2000
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCHRNA4 is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CHRNA4
Synonyms NACRA4
Function After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane permeable to sodium ions.
Cellular Location Postsynaptic cell membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein. Cell membrane; Lipid-anchor
Research Areas
Citations (0)
citation

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References

1.Epilepsy Res. 2014 Dec;108(10):1927-31.2.J Pharmacol Exp Ther. 2014 Mar;348(3):410-20.

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$ 385.00
Cat# AO2581a
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