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ATG4C

Purified Mouse Monoclonal Antibody

     
  • E - ATG4C AO2577a
    Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
    detail
  • WB - ATG4C AO2577a
    Figure 2:Western blot analysis using ATG4C mAb against human ATG4C (AA: 321-458) recombinant protein. (Expected MW is 42.2 kDa)
    detail
  • WB - ATG4C AO2577a
    Figure 3:Western blot analysis using ATG4C mAb against HEK293 (1) and ATG4C (AA: 321-458)-hIgGFc transfected HEK293 (2) cell lysate.
    detail
  • WB - ATG4C AO2577a
    Figure 4:Western blot analysis using ATG4C mouse mAb against HEK293 (1) and MOLT4 (2) cell lysate.
    detail
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IHC, ICC, E
Primary Accession Q96DT6
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 2E10H7
Isotype Mouse IgG1
Calculated MW 52.5kDa
Immunogen Purified recombinant fragment of human ATG4C (AA: 321-458) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide
Additional Information
Gene ID 84938
Other Names APG4C; AUTL1; AUTL3; APG4-C
Dilution E~~ 1/10000
WB~~ 1/500 - 1/2000
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsATG4C is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ATG4C {ECO:0000303|PubMed:21177865, ECO:0000312|HGNC:HGNC:16040}
Function Cysteine protease that plays a key role in autophagy by mediating both proteolytic activation and delipidation of ATG8 family proteins (PubMed:21177865, PubMed:29458288, PubMed:30661429). The protease activity is required for proteolytic activation of ATG8 family proteins: cleaves the C-terminal amino acid of ATG8 proteins MAP1LC3 and GABARAPL2, to reveal a C-terminal glycine (PubMed:21177865). Exposure of the glycine at the C-terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, which is necessary for autophagy (By similarity). In addition to the protease activity, also mediates delipidation of ATG8 family proteins (PubMed:29458288, PubMed:33909989). Catalyzes delipidation of PE-conjugated forms of ATG8 proteins during macroautophagy (PubMed:29458288, PubMed:33909989). Compared to ATG4B, the major protein for proteolytic activation of ATG8 proteins, shows weaker ability to cleave the C-terminal amino acid of ATG8 proteins, while it displays stronger delipidation activity (PubMed:29458288). In contrast to other members of the family, weakly or not involved in phagophore growth during mitophagy (PubMed:33773106).
Cellular Location Cytoplasm {ECO:0000250|UniProtKB:Q8BGE6}.
Research Areas
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References

1.J Biol Chem. 2007 Jun 22;282(25):18573-83.2.J Biol Chem. 2003 Feb 7;278(6):3671-8.

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$ 385.00
Cat# AO2577a
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