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ALDH1A1

Purified Mouse Monoclonal Antibody

     
  • E - ALDH1A1 AO2559a
    Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
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  • WB - ALDH1A1 AO2559a
    Figure 2:Western blot analysis using ALDH1A1 mAb against human ALDH1A1 (AA: 1-110) recombinant protein. (Expected MW is 38.4 kDa)
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  • WB - ALDH1A1 AO2559a
    Figure 3:Western blot analysis using ALDH1A1 mAb against HEK293 (1) and ALDH1A1 (AA: 1-110)-hIgGFc transfected HEK293 (2) cell lysate.
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  • WB - ALDH1A1 AO2559a
    Figure 4:Western blot analysis using ALDH1A1 mouse mAb against HepG2 (1) and A549 (2) cell lysate.
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  •  - ALDH1A1 AO2559a
    Figure 5:Flow cytometric analysis of HeLa cells using ALDH1A1 mouse mAb (green) and negative control (red).
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  • IHC - ALDH1A1 AO2559a
    Figure 6:Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using ALDH1A1 mouse mAb with DAB staining.
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  • IHC - ALDH1A1 AO2559a
    Figure 7:Immunohistochemical analysis of paraffin-embedded stomach cancer tissues using ALDH1A1 mouse mAb with DAB staining.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IHC, ICC, E
Primary Accession P00352
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 2B2G1
Isotype Mouse IgG1
Calculated MW 54.9kDa
Immunogen Purified recombinant fragment of human ALDH1A1 (AA: 1-110) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide
Additional Information
Gene ID 216
Other Names ALDC; ALDH1; HEL-9; HEL12; PUMB1; ALDH11; RALDH1; ALDH-E1; HEL-S-53e
Dilution E~~ 1/10000
WB~~ 1/500 - 1/2000
IHC~~ 1/200 - 1/1000
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsALDH1A1 is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ALDH1A1 (HGNC:402)
Function Cytosolic dehydrogenase that catalyzes the irreversible oxidation of a wide range of aldehydes to their corresponding carboxylic acid (PubMed:12941160, PubMed:15623782, PubMed:17175089, PubMed:19296407, PubMed:25450233, PubMed:26373694). Functions downstream of retinol dehydrogenases and catalyzes the oxidation of retinaldehyde into retinoic acid, the second step in the oxidation of retinol/vitamin A into retinoic acid (By similarity). This pathway is crucial to control the levels of retinol and retinoic acid, two important molecules which excess can be teratogenic and cytotoxic (By similarity). Also oxidizes aldehydes resulting from lipid peroxidation like (E)-4-hydroxynon-2-enal/HNE, malonaldehyde and hexanal that form protein adducts and are highly cytotoxic. By participating for instance to the clearance of (E)-4-hydroxynon-2-enal/HNE in the lens epithelium prevents the formation of HNE-protein adducts and lens opacification (PubMed:12941160, PubMed:15623782, PubMed:19296407). Functions also downstream of fructosamine-3-kinase in the fructosamine degradation pathway by catalyzing the oxidation of 3-deoxyglucosone, the carbohydrate product of fructosamine 3-phosphate decomposition, which is itself a potent glycating agent that may react with lysine and arginine side-chains of proteins (PubMed:17175089). Has also an aminobutyraldehyde dehydrogenase activity and is probably part of an alternative pathway for the biosynthesis of GABA/4-aminobutanoate in midbrain, thereby playing a role in GABAergic synaptic transmission (By similarity).
Cellular Location Cytoplasm, cytosol. Cell projection, axon {ECO:0000250|UniProtKB:P24549}
Tissue Location Expressed by erythrocytes (at protein level).
Research Areas
Citations (0)
citation

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References

1.Oncotarget. 2015 Dec 1;6(38):41360-9.2.Biomark Med. 2015;9(8):777-90.

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$ 385.00
Cat# AO2559a
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