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RUNX3

Purified Mouse Monoclonal Antibody

     
  • E - RUNX3 AO2535a
    Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
    detail
  • WB - RUNX3 AO2535a
    Figure 2:Western blot analysis using RUNX3 mAb against human RUNX3 (AA: 294-429) recombinant protein. (Expected MW is 40 kDa)
    detail
  • WB - RUNX3 AO2535a
    Figure 3:Western blot analysis using RUNX3 mAb against HEK293 (1) and RUNX3 (AA: 294-429)-hIgGFc transfected HEK293 (2) cell lysate.
    detail
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IHC, ICC, E
Primary Accession Q13761
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 8C9B6
Isotype Mouse IgG1
Calculated MW 44.4kDa
Immunogen Purified recombinant fragment of human RUNX3 (AA: 294-429) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide
Additional Information
Gene ID 864
Other Names AML2; CBFA3; PEBP2aC
Dilution E~~ 1/10000
WB~~ 1/500 - 1/2000
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsRUNX3 is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name RUNX3
Synonyms AML2, CBFA3, PEBP2A3
Function Forms the heterodimeric complex core-binding factor (CBF) with CBFB. RUNX members modulate the transcription of their target genes through recognizing the core consensus binding sequence 5'- TGTGGT-3', or very rarely, 5'-TGCGGT-3', within their regulatory regions via their runt domain, while CBFB is a non-DNA-binding regulatory subunit that allosterically enhances the sequence-specific DNA-binding capacity of RUNX. The heterodimers bind to the core site of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, LCK, IL3 and GM-CSF promoters (By similarity). May be involved in the control of cellular proliferation and/or differentiation. In association with ZFHX3, up- regulates CDKN1A promoter activity following TGF-beta stimulation (PubMed:20599712). CBF complexes repress ZBTB7B transcription factor during cytotoxic (CD8+) T cell development. They bind to RUNX-binding sequence within the ZBTB7B locus acting as transcriptional silencer and allowing for cytotoxic T cell differentiation. CBF complexes binding to the transcriptional silencer is essential for recruitment of nuclear protein complexes that catalyze epigenetic modifications to establish epigenetic ZBTB7B silencing (By similarity).
Cellular Location Nucleus {ECO:0000255|PROSITE-ProRule:PRU00399, ECO:0000269|PubMed:20100835, ECO:0000269|PubMed:20599712}. Cytoplasm. Note=The tyrosine phosphorylated form localizes to the cytoplasm. Translocates from the cytoplasm to the nucleus following TGF-beta stimulation
Tissue Location Expressed in gastric cancer tissues (at protein level).
Research Areas
Citations (0)
citation

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References

1.Genet Mol Res. 2015 Dec 1;14(4):15505-10.2.J Pathol. 2015 Dec;237(4):520-31.

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$ 385.00
Cat# AO2535a
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