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ATG7

Purified Mouse Monoclonal Antibody

     
  • E - ATG7 AO2527a
    Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
    detail
  • WB - ATG7 AO2527a
    Figure 2:Western blot analysis using ATG7 mAb against human ATG7 (AA: 558-703) recombinant protein. (Expected MW is 41.9 kDa)
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  • WB - ATG7 AO2527a
    Figure 3:Western blot analysis using ATG7 mAb against HEK293 (1) and ATG7 (AA: 558-703)-hIgGFc transfected HEK293 (2) cell lysate.
    detail
  •  - ATG7 AO2527a
    Figure 4:Flow cytometric analysis of HeLa cells using ATG7 mouse mAb (green) and negative control (red).
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  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IHC, ICC, E
Primary Accession O95352
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 7D3E5
Isotype Mouse IgG2b
Calculated MW 78kDa
Immunogen Purified recombinant fragment of human ATG7 (AA: 558-703) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide
Additional Information
Gene ID 10533
Other Names GSA7; APG7L; APG7-LIKE
Dilution E~~ 1/10000
WB~~ 1/500 - 1/2000
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsATG7 is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ATG7 (HGNC:16935)
Synonyms APG7L
Function E1-like activating enzyme involved in the 2 ubiquitin-like systems required for cytoplasm to vacuole transport (Cvt) and autophagy. Activates ATG12 for its conjugation with ATG5 as well as the ATG8 family proteins for their conjugation with phosphatidylethanolamine. Both systems are needed for the ATG8 association to Cvt vesicles and autophagosomes membranes. Required for autophagic death induced by caspase-8 inhibition. Facilitates LC3-I lipidation with phosphatidylethanolamine to form LC3-II which is found on autophagosomal membranes (PubMed:34161705). Required for mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production. Modulates p53/TP53 activity to regulate cell cycle and survival during metabolic stress. Also plays a key role in the maintenance of axonal homeostasis, the prevention of axonal degeneration, the maintenance of hematopoietic stem cells, the formation of Paneth cell granules, as well as in adipose differentiation. Plays a role in regulating the liver clock and glucose metabolism by mediating the autophagic degradation of CRY1 (clock repressor) in a time-dependent manner (By similarity).
Cellular Location Cytoplasm. Preautophagosomal structure. Note=Localizes also to discrete punctae along the ciliary axoneme and to the base of the ciliary axoneme
Tissue Location Widely expressed, especially in kidney, liver, lymph nodes and bone marrow.
Research Areas
Citations (0)
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References

1.Oncotarget. 2014 Mar 30;5(6):1526-37.2.J Biol Chem. 2012 Oct 12;287(42):35576-88.

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$ 385.00
Cat# AO2527a
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