ASF1B
Purified Mouse Monoclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, IHC, ICC, E |
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Primary Accession | Q9NVP2 |
Reactivity | Human, Mouse, Monkey |
Host | Mouse |
Clonality | Monoclonal |
Clone Names | 6G7G4 |
Isotype | Mouse IgG1 |
Calculated MW | 22.4kDa |
Immunogen | Purified recombinant fragment of human ASF1B (AA: 1-202) expressed in E. Coli. |
Formulation | Purified antibody in PBS with 0.05% sodium azide |
Gene ID | 55723 |
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Other Names | CIA-II |
Dilution | E~~ 1/10000 WB~~ 1/500 - 1/2000 |
Storage | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Precautions | ASF1B is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | ASF1B {ECO:0000303|PubMed:11470414, ECO:0000312|HGNC:HGNC:20996} |
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Function | Histone chaperone that facilitates histone deposition and histone exchange and removal during nucleosome assembly and disassembly (PubMed:11897662, PubMed:14718166, PubMed:15664198, PubMed:16151251, PubMed:21454524, PubMed:26527279). Cooperates with chromatin assembly factor 1 (CAF-1) to promote replication-dependent chromatin assembly (PubMed:11897662, PubMed:14718166, PubMed:15664198, PubMed:16151251). Also involved in the nuclear import of the histone H3-H4 dimer together with importin-4 (IPO4): specifically recognizes and binds newly synthesized histones with the monomethylation of H3 'Lys-9' (H3K9me1) and diacetylation at 'Lys-5' and 'Lys-12' of H4 (H4K5K12ac) marks in the cytosol (PubMed:20953179, PubMed:21454524, PubMed:26527279). Does not participate in replication-independent nucleosome deposition which is mediated by ASF1A and HIRA (PubMed:11897662, PubMed:14718166, PubMed:15664198, PubMed:16151251). Required for gonad development (PubMed:12842904). |
Cellular Location | Nucleus. Cytoplasm, cytosol |
Tissue Location | Highly expressed in testis and at lower levels in colon, small intestine and thymus. |
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Provided below are standard protocols that you may find useful for product applications.
References
1.EMBO J. 2011 Feb 2;30(3):480-93. 2.Mol Cell Biol. 2008 Jun;28(11):3672-85.
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