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TFAP2A

Purified Mouse Monoclonal Antibody

     
  • E - TFAP2A AO2498a
    Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
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  • WB - TFAP2A AO2498a
    Figure 2:Western blot analysis using TFAP2A mAb against human TFAP2A (AA: 105-211) recombinant protein. (Expected MW is 37.5 kDa)
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  • WB - TFAP2A AO2498a
    Figure 3:Western blot analysis using TFAP2A mAb against HEK293 (1) and TFAP2A (AA: 105-211)-hIgGFc transfected HEK293 (2) cell lysate.
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  • WB - TFAP2A AO2498a
    Figure 4:Western blot analysis using TFAP2A mouse mAb against Hela (1), PANC-1 (2), HEK293 (3), and MCF-7 (4) cell lysate.
    detail
  •  - TFAP2A AO2498a
    Figure 7:Flow cytometric analysis of Hela cells using TFAP2A mouse mAb (green) and negative control (red).
    detail
  • ICC - TFAP2A AO2498a
    Figure 5:Immunofluorescence analysis of Hela cells using TFAP2A mouse mAb. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.
    detail
  • ICC - TFAP2A AO2498a
    Figure 6:Immunofluorescence analysis of Hela cells using TFAP2A mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
    detail
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IHC, ICC, E
Primary Accession P05549
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 2H9B5
Isotype Mouse IgG1
Calculated MW 48kDa
Immunogen Purified recombinant fragment of human TFAP2A (AA: 105-211) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide
Additional Information
Gene ID 7020
Other Names AP-2; BOFS; AP2TF; TFAP2; AP-2alpha
Dilution E~~ 1/10000
WB~~ 1/500 - 1/2000
ICC~~ 1/200 - 1/1000
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsTFAP2A is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name TFAP2A
Synonyms AP2TF, TFAP2
Function Sequence-specific DNA-binding protein that interacts with inducible viral and cellular enhancer elements to regulate transcription of selected genes. AP-2 factors bind to the consensus sequence 5'-GCCNNNGGC-3' and activate genes involved in a large spectrum of important biological functions including proper eye, face, body wall, limb and neural tube development. They also suppress a number of genes including MCAM/MUC18, C/EBP alpha and MYC. AP-2-alpha is the only AP-2 protein required for early morphogenesis of the lens vesicle. Together with the CITED2 coactivator, stimulates the PITX2 P1 promoter transcription activation. Associates with chromatin to the PITX2 P1 promoter region.
Cellular Location Nucleus.
Citations (0)
citation

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References

1.Int J Clin Exp Pathol. 2014 Nov 26;7(12):8666-74.2.Hum Pathol. 2012 Nov;43(11):1866-74.

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$ 385.00
Cat# AO2498a
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