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> home > Products > Primary Antibodies > Antibody Collections > Ubiquitin E3 Ligases (RING Domain) Antibodies > Mouse Monoclonal Antibody to UHRF1
Mouse Monoclonal Antibody to UHRF1
Purified Mouse Monoclonal Antibody
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Application 
| WB, IHC, FC, ICC, E |
|---|---|
| Primary Accession | Q96T88 |
| Reactivity | Human |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone Names | 1A1B1 |
| Isotype | Mouse IgG2b |
| Calculated MW | 89.8kDa |
| Description | This gene encodes a member of a subfamily of RING-finger type E3 ubiquitin ligases. The protein binds to specific DNA sequences, and recruits a histone deacetylase to regulate gene expression. Its expression peaks at late G1 phase and continues during G2 and M phases of the cell cycle. It plays a major role in the G1/S transition by regulating topoisomerase IIalpha and retinoblastoma gene expression, and functions in the p53-dependent DNA damage checkpoint. It is regarded as a hub protein for the integration of epigenetic information. This gene is up-regulated in various cancers, and it is therefore considered to be a therapeutic target. Multiple transcript variants encoding different isoforms have been found for this gene. A related pseudogene exists on chromosome 12.; |
| Immunogen | Purified recombinant fragment of human UHRF1 (AA: 616-755) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
| Application Note | ELISA: 1/10000 WB: 1/500 - 1/2000 IHC: 1/200 - 1/1000 ICC: 1/200 - 1/1000 FCM: 1/200 - 1/400 |
| Gene ID | 29128 |
|---|---|
| Other Names | Np95; hNP95; ICBP90; RNF106; TDRD22; hUHRF1; huNp95 |
| Storage | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | Mouse Monoclonal Antibody to UHRF1 is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | UHRF1 |
|---|---|
| Synonyms | ICBP90, NP95, RNF106 |
| Function | Multidomain protein that acts as a key epigenetic regulator by bridging DNA methylation and chromatin modification. Specifically recognizes and binds hemimethylated DNA at replication forks via its YDG domain and recruits DNMT1 methyltransferase to ensure faithful propagation of the DNA methylation patterns through DNA replication. In addition to its role in maintenance of DNA methylation, also plays a key role in chromatin modification: through its tudor-like regions and PHD-type zinc fingers, specifically recognizes and binds histone H3 trimethylated at 'Lys-9' (H3K9me3) and unmethylated at 'Arg-2' (H3R2me0), respectively, and recruits chromatin proteins. Enriched in pericentric heterochromatin where it recruits different chromatin modifiers required for this chromatin replication. Also localizes to euchromatic regions where it negatively regulates transcription possibly by impacting DNA methylation and histone modifications. Has E3 ubiquitin-protein ligase activity by mediating the ubiquitination of target proteins such as histone H3 and PML. It is still unclear how E3 ubiquitin-protein ligase activity is related to its role in chromatin in vivo. Plays a role in DNA repair by cooperating with UHRF2 to ensure recruitment of FANCD2 to interstrand cross-links (ICLs) leading to FANCD2 activation. Acts as a critical player of proper spindle architecture by catalyzing the 'Lys-63'-linked ubiquitination of KIF11, thereby controlling KIF11 localization on the spindle (PubMed:37728657). |
| Cellular Location | Nucleus {ECO:0000255|PROSITE-ProRule:PRU00358, ECO:0000269|PubMed:10646863, ECO:0000269|PubMed:17673620, ECO:0000269|PubMed:17967883, ECO:0000269|PubMed:19056828, ECO:0000269|PubMed:21777816, ECO:0000269|PubMed:30335751} Note=Associated, through the YDG domain (also called SRA domain), with replicating DNA from early to late S phase, including at replicating pericentric heterochromatin (By similarity). Also localizes to euchromatic regions. In non-S-phase cells, homogenously distributed through the nucleus (By similarity). {ECO:0000250|UniProtKB:Q8VDF2} |
| Tissue Location | Expressed in thymus, bone marrow, testis, lung and heart. Overexpressed in breast cancer. |
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Application Protocols
Provided below are standard protocols that you may find useful for product applications.
References
1.Biomarkers. 2015;20(3):183-8. ; 2.Med Oncol. 2013 Dec;30(4):613. ;
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$ 385.00
Cat# AO2454a
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