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Mouse Monoclonal Antibody to IDH1

Purified Mouse Monoclonal Antibody

     
  • E - Mouse Monoclonal Antibody to IDH1 AO2435a
    Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
    detail
  • WB - Mouse Monoclonal Antibody to IDH1 AO2435a
    Western blot analysis using IDH1 mAb against human IDH1 (AA: 156-298) recombinant protein. (Expected MW is 41.8 kDa)
    detail
  • WB - Mouse Monoclonal Antibody to IDH1 AO2435a
    Western blot analysis using IDH1 mAb against HEK293 (1) and IDH1 (AA: 156-298)-hIgGFc transfected HEK293 (2) cell lysate.
    detail
  • WB - Mouse Monoclonal Antibody to IDH1 AO2435a
    Western blot analysis using IDH1 mouse mAb against HepG2 (1), NIH/3T3 (2), C2C12 (3), COS7 (4), and SW480 (5) cell lysate.
    detail
  • FC - Mouse Monoclonal Antibody to IDH1 AO2435a
    Flow cytometric analysis of Hela cells using IDH1 mouse mAb (green) and negative control (red).
    detail
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, FC, E
Primary Accession O75874
Reactivity Human, Mouse, Monkey
Host Mouse
Clonality Monoclonal
Clone Names 4A4A8
Isotype Mouse IgG1
Calculated MW 46.7kDa
Description Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP(+)-dependent isozyme is a homodimer. The protein encoded by this gene is the NADP(+)-dependent isocitrate dehydrogenase found in the cytoplasm and peroxisomes. It contains the PTS-1 peroxisomal targeting signal sequence. The presence of this enzyme in peroxisomes suggests roles in the regeneration of NADPH for intraperoxisomal reductions, such as the conversion of 2, 4-dienoyl-CoAs to 3-enoyl-CoAs, as well as in peroxisomal reactions that consume 2-oxoglutarate, namely the alpha-hydroxylation of phytanic acid. The cytoplasmic enzyme serves a significant role in cytoplasmic NADPH production. Alternatively spliced transcript variants encoding the same protein have been found for this gene.;
Immunogen Purified recombinant fragment of human IDH1 (AA: 156-298) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide
Application Note ELISA: 1/10000
WB: 1/500 - 1/2000
FCM: 1/200 - 1/400
Additional Information
Gene ID 3417
Other Names IDH; IDP; IDCD; IDPC; PICD; HEL-216; HEL-S-26
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsMouse Monoclonal Antibody to IDH1 is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name IDH1
Synonyms PICD
Function Catalyzes the NADP(+)-dependent oxidative decarboxylation of isocitrate (D-threo-isocitrate) to 2-ketoglutarate (2-oxoglutarate), which is required by other enzymes such as the phytanoyl-CoA dioxygenase (PubMed:10521434, PubMed:19935646). Plays a critical role in the generation of NADPH, an important cofactor in many biosynthesis pathways (PubMed:10521434). May act as a corneal epithelial crystallin and may be involved in maintaining corneal epithelial transparency (By similarity).
Cellular Location Cytoplasm, cytosol. Peroxisome
Research Areas
Citations (0)
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References

1.Cancer Cell. 2015 Dec 14;28(6):773-84. ; 2.Int J Cancer. 2015 Sep 1;137(5):1058-65. ;

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$ 385.00
Cat# AO2435a
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