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> home > Products > Primary Antibodies > Antibody Collections > Host-virus interaction > Mouse Monoclonal Antibody to BNIP3
Mouse Monoclonal Antibody to BNIP3
Purified Mouse Monoclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, FC, ICC, E |
|---|---|
| Primary Accession | Q12983 |
| Reactivity | Human |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone Names | 4E11F4 |
| Isotype | Mouse IgG1 |
| Calculated MW | 21.5kDa |
| Description | This gene is encodes a mitochondrial protein that contains a BH3 domain and acts as a pro-apoptotic factor. The encoded protein interacts with anti-apoptotic proteins, including the E1B 19 kDa protein and Bcl2. This gene is silenced in tumors by DNA methylation.; |
| Immunogen | Purified recombinant fragment of human BNIP3 (AA: 50-155) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
| Application Note | ELISA: 1/10000 WB: 1/500 - 1/2000 ICC: 1/200 - 1/1000 FCM: 1/200 - 1/400 |
| Gene ID | 664 |
|---|---|
| Other Names | NIP3 |
| Storage | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | Mouse Monoclonal Antibody to BNIP3 is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | BNIP3 (HGNC:1084) |
|---|---|
| Synonyms | NIP3 |
| Function | Apoptosis-inducing protein that can overcome BCL2 suppression. May play a role in repartitioning calcium between the two major intracellular calcium stores in association with BCL2. Involved in mitochondrial quality control via its interaction with SPATA18/MIEAP: in response to mitochondrial damage, participates in mitochondrial protein catabolic process (also named MALM) leading to the degradation of damaged proteins inside mitochondria. The physical interaction of SPATA18/MIEAP, BNIP3 and BNIP3L/NIX at the mitochondrial outer membrane regulates the opening of a pore in the mitochondrial double membrane in order to mediate the translocation of lysosomal proteins from the cytoplasm to the mitochondrial matrix. Plays an important role in the calprotectin (S100A8/A9)-induced cell death pathway. |
| Cellular Location | Mitochondrion. Mitochondrion outer membrane; Single-pass membrane protein. Note=Coexpression with the EIB 19-kDa protein results in a shift in NIP3 localization pattern to the nuclear envelope. Colocalizes with ACAA2 in the mitochondria. Colocalizes with SPATA18 at the mitochondrion outer membrane |
Research Areas
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Application Protocols
Provided below are standard protocols that you may find useful for product applications.
References
1.Tumour Biol. 2015 Jun;36(6):4731-40. ; 2.PLoS One. 2014 May 13;9(5):e96733. ;
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$ 385.00
Cat# AO2407a
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