Mouse Monoclonal Antibody to B3GAT1
Purified Mouse Monoclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application ![]()
| WB, FC, E |
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Primary Accession | Q9P2W7 |
Reactivity | Human |
Host | Mouse |
Clonality | Monoclonal |
Clone Names | 3B8C12 |
Isotype | Mouse IgG1 |
Calculated MW | 38.2kDa |
Description | The protein encoded by this gene is a member of the glucuronyltransferase gene family. These enzymes exhibit strict acceptor specificity, recognizing nonreducing terminal sugars and their anomeric linkages. This gene product functions as the key enzyme in a glucuronyl transfer reaction during the biosynthesis of the carbohydrate epitope HNK-1 (human natural killer-1, also known as CD57 and LEU7). Alternate transcriptional splice variants have been characterized.; |
Immunogen | Purified recombinant fragment of human B3GAT1 (AA: 193-334) expressed in E. Coli. |
Formulation | Purified antibody in PBS with 0.05% sodium azide |
Application Note | ELISA: 1/10000 WB: 1/500 - 1/2000 FCM: 1/200 - 1/400 |
Gene ID | 27087 |
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Other Names | NK1; CD57; HNK1; LEU7; NK-1; GLCATP; GLCUATP |
Storage | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Precautions | Mouse Monoclonal Antibody to B3GAT1 is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | B3GAT1 (HGNC:921) |
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Synonyms | GLCATP |
Function | Involved in the biosynthesis of L2/HNK-1 carbohydrate epitope on glycoproteins. Can also play a role in glycosaminoglycan biosynthesis. Substrates include asialo-orosomucoid (ASOR), asialo- fetuin, and asialo-neural cell adhesion molecule. Requires sphingomyelin for activity: stearoyl-sphingomyelin was the most effective, followed by palmitoyl-sphingomyelin and lignoceroyl- sphingomyelin. Activity was demonstrated only for sphingomyelin with a saturated fatty acid and not for that with an unsaturated fatty acid, regardless of the length of the acyl group. |
Cellular Location | [Isoform 1]: Golgi apparatus membrane {ECO:0000250|UniProtKB:O35789}; Single-pass type II membrane protein {ECO:0000250|UniProtKB:O35789}. Secreted {ECO:0000250|UniProtKB:O35789} |
Tissue Location | Mainly expressed in the brain. |
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Provided below are standard protocols that you may find useful for product applications.
References
1.Biomed Res Int. 2014;2014:356427. ; 2.PLoS One. 2013;8(2):e52144.;
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