Mouse Monoclonal Antibody to PGRMC1
Purified Mouse Monoclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, IHC, FC, ICC, E |
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Primary Accession | O00264 |
Reactivity | Human |
Host | Mouse |
Clonality | Monoclonal |
Clone Names | 7G11G8 |
Isotype | Mouse IgG1 |
Calculated MW | 21.7kDa |
Description | This gene encodes a putative membrane-associated progesterone steroid receptor. The protein is expressed predominantly in the liver and kidney.; |
Immunogen | Purified recombinant fragment of human PGRMC1 (AA: 1-195) expressed in E. Coli. |
Formulation | Purified antibody in PBS with 0.05% sodium azide |
Application Note | ELISA: 1/10000 WB: 1/500 - 1/2000 IHC: 1/200 - 1/1000 ICC: 1/200 - 1/1000 FCM: 1/200 - 1/400 |
Gene ID | 10857 |
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Other Names | MPR; HPR6.6 |
Storage | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Precautions | Mouse Monoclonal Antibody to PGRMC1 is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | PGRMC1 (HGNC:16090) |
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Function | Component of a progesterone-binding protein complex (PubMed:28396637). Binds progesterone (PubMed:25675345). Has many reported cellular functions (heme homeostasis, interaction with CYPs). Required for the maintenance of uterine histoarchitecture and normal female reproductive lifespan (By similarity). Intracellular heme chaperone. Regulates heme synthesis via interactions with FECH and acts as a heme donor for at least some hemoproteins (PubMed:27599036). Forms a ternary complex with TMEM97 receptor and low density lipid receptor/LDLR, which increases LDLR-mediated LDL lipoprotein internalization (PubMed:30443021). |
Cellular Location | Microsome membrane {ECO:0000250|UniProtKB:Q95250}; Single-pass membrane protein. Smooth endoplasmic reticulum membrane; Single-pass membrane protein. Mitochondrion outer membrane {ECO:0000250|UniProtKB:O55022}; Single-pass membrane protein; Extracellular side {ECO:0000250|UniProtKB:O55022} Secreted Note=Localized at cell membrane, probably in lipid rafts, in serum- starved conditions. |
Tissue Location | Detected in urine (at protein level) (PubMed:36213313, PubMed:37453717). Expressed by endometrial glands and stroma (at protein level) (PubMed:23793472). Widely expressed, with highest expression in liver and kidney. |
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Provided below are standard protocols that you may find useful for product applications.
References
1.Cancer Lett. 2015 Jan 28;356(2 Pt B):434-42. ; 2.Nan Fang Yi Ke Da Xue Xue Bao. 2012 May;32(5):635-8. ;
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