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SERPINA1 Antibody

Purified Mouse Monoclonal Antibody

     
  • E - SERPINA1 Antibody AO2238a

    Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

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  • WB - SERPINA1 Antibody AO2238a

    Figure 2:Western blot analysis using SERPINA1 mAb against human SERPINA1 (AA: 23-237) recombinant protein. (Expected MW is 50.3 kDa)

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  • WB - SERPINA1 Antibody AO2238a

    Figure 3:Western blot analysis using SERPINA1 mAb against HEK293 (1) and SERPINA1 (AA: 23-237)-hIgGFc transfected HEK293 (2) cell lysate.

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  • IF - SERPINA1 Antibody AO2238a

    Figure 4:Immunofluorescence analysis of GC-7901 cells using SERPINA1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

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  • IF - SERPINA1 Antibody AO2238a

    Figure 5:Immunofluorescence analysis of HepG2 cells using SERPINA1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

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  • FC - SERPINA1 Antibody AO2238a

    Figure 6:Flow cytometric analysis of Hela cells using SERPINA1 mouse mAb (green) and negative control (red).

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  • IHC - SERPINA1 Antibody AO2238a

    Figure 7:Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using SERPINA1 mouse mAb with DAB staining.

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  • IHC - SERPINA1 Antibody AO2238a

    Figure 8:Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using SERPINA1 mouse mAb with DAB staining.

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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IHC, FC, ICC, E
Primary Accession P01009
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 5D1D2
Isotype IgG1
Calculated MW 46.7kDa
Description The protein encoded by this gene is secreted and is a serine protease inhibitor whose targets include elastase, plasmin, thrombin, trypsin, chymotrypsin, and plasminogen activator. Defects in this gene can cause emphysema or liver disease. Several transcript variants encoding the same protein have been found for this gene.
Immunogen Purified recombinant fragment of human SERPINA1 (AA: 23-237) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide
Additional Information
Gene ID 5265
Other Names Alpha-1-antitrypsin, Alpha-1 protease inhibitor, Alpha-1-antiproteinase, Serpin A1, Short peptide from AAT, SPAAT, SERPINA1, AAT, PI
Dilution E~~1/10000
WB~~1/500 - 1/2000
IF~~1/200 - 1/1000
FC~~1/200 - 1/400
IHC~~1/200 - 1/1000
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsSERPINA1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name SERPINA1 (HGNC:8941)
Synonyms AAT, PI
Function Inhibitor of serine proteases. Its primary target is elastase, but it also has a moderate affinity for plasmin and thrombin. Irreversibly inhibits trypsin, chymotrypsin and plasminogen activator. The aberrant form inhibits insulin-induced NO synthesis in platelets, decreases coagulation time and has proteolytic activity against insulin and plasmin.
Cellular Location Secreted. Endoplasmic reticulum. Note=The S and Z allele are not secreted effectively and accumulate intracellularly in the endoplasmic reticulum
Tissue Location Ubiquitous. Expressed in leukocytes and plasma.
Research Areas
Citations (0)
citation

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References

1.PLoS One. 2012;7(8):e42728. 2.Electrophoresis. 2012 Jul;33(14):2130-7.

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$ 385.00
Cat# AO2238a
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