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MECP2 Antibody

Purified Mouse Monoclonal Antibody

     
  • E - MECP2 Antibody AO2163a

    Black line: Control Antigen (100 ng);
    Purple line: Antigen(10ng);
    Blue line: Antigen (50 ng);
    Red line: Antigen (100 ng);

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  • WB - MECP2 Antibody AO2163a

    Figure 2:Western blot analysis using MECP2 mAb against human MECP2 (AA: 7-148) recombinant protein. (Expected MW is 41.7 kDa)

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  • WB - MECP2 Antibody AO2163a

    Figure 3:Western blot analysis using MECP2 mAb against HEK293 (1) and MECP2 (AA: 7-148)-hIgGFc transfected HEK293 (2) cell lysate.

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  • WB - MECP2 Antibody AO2163a

    Figure 4:Western blot analysis using MECP2 mouse mAb against A431 (1) and MCF-7 (2) cell lysate.

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  • IF - MECP2 Antibody AO2163a

    Figure 5:Immunofluorescence analysis of Hela cells using MECP2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

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  • FC - MECP2 Antibody AO2163a

    Figure 6:Flow cytometric analysis of Hela cells using MECP2 mouse mAb (green) and negative control (red).

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  • IHC - MECP2 Antibody AO2163a

    Figure 7:Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using MECP2 mouse mAb with DAB staining.

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  • IHC - MECP2 Antibody AO2163a

    Figure 8:Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using MECP2 mouse mAb with DAB staining.

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  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IHC, FC, ICC, E
Primary Accession P51608
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 8H4A5B9
Isotype IgG1
Calculated MW 52.4kDa
Description DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MECP2, MBD1 and MBD2 can also repress transcription from methylated gene promoters. In contrast to other MBD family members, MECP2 is X-linked and subject to X inactivation. MECP2 is dispensible in stem cells, but is essential for embryonic development. MECP2 gene mutations are the cause of most cases of Rett syndrome, a progressive neurologic developmental disorder and one of the most common causes of mental retardation in females.
Immunogen Purified recombinant fragment of human MECP2 (AA: 7-148) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide
Additional Information
Gene ID 4204
Other Names Methyl-CpG-binding protein 2, MeCp-2 protein, MeCp2, MECP2
Dilution E~~1/10000
WB~~1/500 - 1/2000
IF~~1/200 - 1/1000
FC~~1/200 - 1/400
IHC~~1/200 - 1/1000
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsMECP2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name MECP2
Function Chromosomal protein that binds to methylated DNA. It can bind specifically to a single methyl-CpG pair. It is not influenced by sequences flanking the methyl-CpGs. Mediates transcriptional repression through interaction with histone deacetylase and the corepressor SIN3A. Binds both 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC)- containing DNA, with a preference for 5-methylcytosine (5mC).
Cellular Location Nucleus {ECO:0000250|UniProtKB:Q9Z2D6}. Note=Colocalized with methyl-CpG in the genome. Colocalized with TBL1X to the heterochromatin foci.
Tissue Location Present in all adult somatic tissues tested.
Research Areas
Citations (0)
citation

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References

1.J Pediatr Surg. 2013 Oct;48(10):2099-105.2.Cell Res. 2013 Nov;23(11):1244-6.

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$ 385.00
Cat# AO2163a
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