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HIST2H4A(20Me) Antibody

Purified Mouse Monoclonal Antibody

     
  • E - HIST2H4A(20Me) Antibody AO2144a

    Black line: Control Antigen (100 ng);
    Purple line: Antigen(10ng);
    Blue line: Antigen (50 ng);
    Red line: Antigen (100 ng);

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  • IF - HIST2H4A(20Me) Antibody AO2144a

    Figure 2:Immunofluorescence analysis of HeLa cells . Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.

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  • IF - HIST2H4A(20Me) Antibody AO2144a

    Figure 3:Immunofluorescence analysis of HeLa cells using HIST2H4A(20Me) mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

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  • IHC - HIST2H4A(20Me) Antibody AO2144a

    Figure 4:Immunohistochemical analysis of paraffin-embedded esophageal cancer tissues using HIST2H4A(20Me) mouse mAb with DAB staining.

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  • IHC - HIST2H4A(20Me) Antibody AO2144a

    Figure 5:Immunohistochemical analysis of paraffin-embedded colon cancer tissues using HIST2H4A(20Me) mouse mAb with DAB staining.

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  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
IHC, ICC, E
Primary Accession P62805
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 3E7D9
Isotype IgG1
Calculated MW 11.4kDa
Description Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H4 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in a histone cluster on chromosome 1. This gene is one of four histone genes in the cluster that are duplicated; this record represents the centromeric copy.
Immunogen Synthesized peptide of human HIST2H4A ( AA: GGAKRHRK(Me)VLRDNIQ ) .
Formulation Purified antibody in PBS with 0.05% sodium azide
Additional Information
Gene ID 121504;554313;8294;8359;8360;8361;8362;8363;8364;8365;8366;8367;8368;8370
Dilution E~~1/10000
IF~~1/200 - 1/1000
IHC~~1/200 - 1/1000
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsHIST2H4A(20Me) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name H4C1
Synonyms H4/A, H4FA, HIST1H4A
Function Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Cellular Location Nucleus. Chromosome.
Citations (0)
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References

1.J Virol. 2011 Dec;85(24):13234-52.2.Mol Cell Biol. 2003 Feb;23(4):1460-9.

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$ 385.00
Cat# AO2144a
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