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Rab3a Antibody

Purified Mouse Monoclonal Antibody

     
  • E - Rab3a Antibody AO2128a

    Black line: Control Antigen (100 ng);
    Purple line: Antigen(10ng);
    Blue line: Antigen (50 ng);
    Red line: Antigen (100 ng);

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  • WB - Rab3a Antibody AO2128a

    Figure 2:Western blot analysis using Rab3a mAb against human Rab3a (AA: 1-220) recombinant protein. (Expected MW is 50.9 kDa)

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  • WB - Rab3a Antibody AO2128a

    Figure 3:Western blot analysis using Rab3a mAb against HEK293 (1) and Rab3a (AA: 1-220)-hIgGFc transfected HEK293 (2) cell lysate.

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  • FC - Rab3a Antibody AO2128a

    Figure 4:Flow cytometric analysis of HeLa cells using Rab3a mouse mAb (green) and negative control (red).

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  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, FC, E
Primary Accession P20336
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 6D1A12
Isotype IgG1
Calculated MW 25kDa
Description RAB3A (RAB3A, member RAS oncogene family) is a protein-coding gene. Diseases associated with RAB3A include choroideremia. GO annotations related to this gene include protein C-terminus binding and GTP binding. An important paralog of this gene is RAB10.
Immunogen Purified recombinant fragment of human Rab3a (AA: 1-220) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide
Additional Information
Gene ID 5864
Other Names Ras-related protein Rab-3A, RAB3A
Dilution E~~1/10000
WB~~1/500 - 1/2000
FC~~1/200 - 1/400
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsRab3a Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name RAB3A
Function Small GTP-binding protein that plays a central role in regulated exocytosis and secretion. Controls the recruitment, tethering and docking of secretory vesicles to the plasma membrane (By similarity). Upon stimulation, switches to its active GTP-bound form, cycles to vesicles and recruits effectors such as RIMS1, RIMS2, Rabphilin-3A/RPH3A, RPH3AL or SYTL4 to help the docking of vesicules onto the plasma membrane (By similarity). Upon GTP hydrolysis by GTPase-activating protein, dissociates from the vesicle membrane allowing the exocytosis to proceed (By similarity). Stimulates insulin secretion through interaction with RIMS2 or RPH3AL effectors in pancreatic beta cells (By similarity). Regulates calcium-dependent lysosome exocytosis and plasma membrane repair (PMR) via the interaction with 2 effectors, SYTL4 and myosin-9/MYH9 (PubMed:27325790). Acts as a positive regulator of acrosome content secretion in sperm cells by interacting with RIMS1 (PubMed:22248876, PubMed:30599141). Also plays a role in the regulation of dopamine release by interacting with synaptotagmin I/SYT (By similarity). Interacts with MADD (via uDENN domain); the GTP-bound form is preferred for interaction (By similarity).
Cellular Location Cytoplasm, cytosol {ECO:0000250|UniProtKB:P63012}. Lysosome Cytoplasmic vesicle, secretory vesicle {ECO:0000250|UniProtKB:P63012} Cell projection, axon {ECO:0000250|UniProtKB:P63011}. Cell membrane; Lipid-anchor; Cytoplasmic side. Presynapse {ECO:0000250|UniProtKB:P63011}. Postsynapse {ECO:0000250|UniProtKB:P63011}. Note=Cycles between a vesicle- associated GTP-bound form and a cytosolic GDP-bound form {ECO:0000250|UniProtKB:P63012}
Tissue Location Specifically expressed in brain.
Research Areas
Citations (0)
citation

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References

1.Mol Biol Rep. 2014 Jun;41(6):3951-9. 2.FASEB J. 2007 Dec;21(14):4121-30.

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$ 385.00
Cat# AO2128a
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