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MMP14 Antibody

Purified Mouse Monoclonal Antibody

     
  • E - MMP14 Antibody AO2127a

    Black line: Control Antigen (100 ng);
    Purple line: Antigen(10ng);
    Blue line: Antigen (50 ng);
    Red line: Antigen (100 ng);

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  • WB - MMP14 Antibody AO2127a

    Figure 2:Western blot analysis using MMP14 mAb against human MMP14 (AA: 112-246) recombinant protein. (Expected MW is 41.3 kDa)

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  • WB - MMP14 Antibody AO2127a

    Figure 3:Western blot analysis using MMP14 mAb against HEK293 (1) and MMP14 (AA: 112-246)-hIgGFc transfected HEK293 (2) cell lysate.

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  • FC - MMP14 Antibody AO2127a

    Figure 4:Flow cytometric analysis of HT1080 cells using MMP14 mouse mAb (green) and negative control (red).

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  • IHC - MMP14 Antibody AO2127a

    Figure 5:Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using MMP14 mouse mAb with DAB staining.

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  • IHC - MMP14 Antibody AO2127a

    Figure 6:Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using MMP14 mouse mAb with DAB staining.

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  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IHC, FC, E
Primary Accession P50281
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 6A11D1
Isotype IgG1
Calculated MW 65.9kDa
Description Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. However, the protein encoded by this gene is a member of the membrane-type MMP (MT-MMP) subfamily; each member of this subfamily contains a potential transmembrane domain suggesting that these proteins are expressed at the cell surface rather than secreted. This protein activates MMP2 protein, and this activity may be involved in tumor invasion.
Immunogen Purified recombinant fragment of human MMP14 (AA: 112-246) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide
Additional Information
Gene ID 4323
Other Names Matrix metalloproteinase-14, MMP-14, 3.4.24.80, MMP-X1, Membrane-type matrix metalloproteinase 1, MT-MMP 1, MTMMP1, Membrane-type-1 matrix metalloproteinase, MT1-MMP, MT1MMP, MMP14
Dilution E~~1/10000
WB~~1/500 - 1/2000
FC~~1/200 - 1/400
IHC~~1/200 - 1/1000
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsMMP14 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name MMP14
Function Endopeptidase that degrades various components of the extracellular matrix such as collagen (PubMed:8015608). Essential for pericellular collagenolysis and modeling of skeletal and extraskeletal connective tissues during development (By similarity). Activates progelatinase A/MMP2, thereby acting as a positive regulator of cell growth and migration (PubMed:22065321, PubMed:8015608). Involved in the formation of the fibrovascular tissues in association with pro-MMP2 (PubMed:12714657, PubMed:22065321). May be involved in actin cytoskeleton reorganization by cleaving PTK7 (PubMed:20837484). Acts as a regulator of Notch signaling by mediating cleavage and inhibition of DLL1 (PubMed:21572390). Cleaves ADGRB1 to release vasculostatin-40 which inhibits angiogenesis (PubMed:22330140). Acts as a negative regulator of the GDF15-GFRAL aversive response by mediating cleavage and inactivation of GFRAL (PubMed:35177851).
Cellular Location Cell membrane; Single-pass type I membrane protein. Melanosome. Cytoplasm Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV (PubMed:17081065). Forms a complex with BST2 and localizes to the cytoplasm (PubMed:17081065)
Tissue Location Expressed in stromal cells of colon, breast, and head and neck. Expressed in lung tumors.
Research Areas
Citations (0)
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References

1.Cancer Med. 2013 Aug;2(4):457-67.2.BMC Cancer. 2013 Feb 10;13:74.

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$ 385.00
Cat# AO2127a
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