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CD168 Antibody

Purified Mouse Monoclonal Antibody

     
  • E - CD168 Antibody AO2068a

    Black line: Control Antigen (100 ng);
    Purple line: Antigen(10ng);
    Blue line: Antigen (50 ng);
    Red line: Antigen (100 ng);

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  • WB - CD168 Antibody AO2068a

    Figure 2:Western blot analysis using CD168 mAb against human CD168 (AA: 306-497) recombinant protein. (Expected MW is 48.3 kDa)

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  • WB - CD168 Antibody AO2068a

    Figure 3:Western blot analysis using CD168 mAb against HEK293 (1) and CD168 (AA: 306-497)-hIgGFc transfected HEK293 (2) cell lysate.

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  • FC - CD168 Antibody AO2068a

    Figure 4:Flow cytometric analysis of Hela cells using CD168 mouse mAb (green) and negative control (red).

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  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, FC, E
Primary Accession O75330
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 2F2C9
Isotype IgG1
Calculated MW 84.1kDa
Description The protein encoded by this gene is involved in cell motility. It is expressed in breast tissue and together with other proteins, it forms a complex with BRCA1 and BRCA2, thus is potentially associated with higher risk of breast cancer. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene.
Immunogen Purified recombinant fragment of human CD168 (AA: 306-497 ) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide
Additional Information
Gene ID 3161
Other Names Hyaluronan mediated motility receptor, Intracellular hyaluronic acid-binding protein, Receptor for hyaluronan-mediated motility, CD168, HMMR, IHABP, RHAMM
Dilution E~~1/10000
WB~~1/500 - 1/2000
FC~~1/200 - 1/400
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCD168 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name HMMR
Synonyms IHABP, RHAMM
Function Receptor for hyaluronic acid (HA) (By similarity). Involved in cell motility (By similarity). When hyaluronan binds to HMMR, the phosphorylation of a number of proteins, including PTK2/FAK1 occurs. May also be involved in cellular transformation and metastasis formation, and in regulating extracellular-regulated kinase (ERK) activity. May act as a regulator of adipogenisis (By similarity).
Cellular Location Cell surface {ECO:0000250|UniProtKB:Q00547}. Cytoplasm {ECO:0000250|UniProtKB:Q00547}. Cytoplasm, cytoskeleton, spindle {ECO:0000250|UniProtKB:Q00547}
Tissue Location Expressed in testis (PubMed:22965910). Expressed in the breast (PubMed:8890751).
Research Areas
Citations (0)
citation

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References

1.PLoS One. 2013 Sep 17;8(9):e75681.2.BMC Cancer. 2011 Mar 24;11:106.

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$ 385.00
Cat# AO2068a
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