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TFAP2A Antibody

Purified Mouse Monoclonal Antibody

     
  • E - TFAP2A Antibody AO2053a

    Black line: Control Antigen (100 ng);
    Purple line: Antigen(10ng);
    Blue line: Antigen (50 ng);
    Red line: Antigen (100 ng);

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  • WB - TFAP2A Antibody AO2053a

    Figure 2:Western blot analysis using TFAP2A mAb against human TFAP2A (AA: 1-100) recombinant protein. (Expected MW is 37 kDa)

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  • WB - TFAP2A Antibody AO2053a

    Figure 3:Western blot analysis using TFAP2A mAb against HEK293 (1) and TFAP2A (AA: 1-100)-hIgGFc transfected HEK293 (2) cell lysate.

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  • WB - TFAP2A Antibody AO2053a

    Figure 4:Western blot analysis using TFAP2A mouse mAb against Hela (1), MCF-7 (2), Cos7 (3), A431 (4), HCT116 (5), NIH/3T3 (6), and PC12 (7) cell lysate.

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  • FC - TFAP2A Antibody AO2053a

    Figure 5:Flow cytometric analysis of Hela cells using TFAP2A mouse mAb (green) and negative control (red).

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  • IHC - TFAP2A Antibody AO2053a

    Figure 6:Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using TFAP2A mouse mAb with DAB staining.

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  • IHC - TFAP2A Antibody AO2053a

    Figure 7:Immunohistochemical analysis of paraffin-embedded TFAP2A tissues using endometrial cancer mouse mAb with DAB staining.

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  • FC - TFAP2A Antibody AO2053a

    Figure 8:Flow cytometric analysis of MCF-7 cells using TFAP2A mouse mAb (green) and negative control (red).

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  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IHC, FC, E
Primary Accession P05549
Reactivity Human, Mouse, Rat, Monkey
Host Mouse
Clonality Monoclonal
Clone Names 7D2B5
Isotype IgG1
Calculated MW 48kDa
Description The protein encoded by this gene is a transcription factor that binds the consensus sequence 5'-GCCNNNGGC-3'. The encoded protein functions as either a homodimer or as a heterodimer with similar family members. This protein activates the transcription of some genes while inhibiting the transcription of others. Defects in this gene are a cause of branchiooculofacial syndrome (BOFS). Three transcript variants encoding different isoforms have been found for this gene.
Immunogen Purified recombinant fragment of human TFAP2A (AA: 1-100) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide
Additional Information
Gene ID 7020
Other Names Transcription factor AP-2-alpha, AP2-alpha, AP-2 transcription factor, Activating enhancer-binding protein 2-alpha, Activator protein 2, AP-2, TFAP2A, AP2TF, TFAP2
Dilution E~~1/10000
WB~~1/500 - 1/2000
FC~~1/200 - 1/400
IHC~~1/200 - 1/1000
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsTFAP2A Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name TFAP2A
Synonyms AP2TF, TFAP2
Function Sequence-specific DNA-binding protein that interacts with inducible viral and cellular enhancer elements to regulate transcription of selected genes. AP-2 factors bind to the consensus sequence 5'-GCCNNNGGC-3' and activate genes involved in a large spectrum of important biological functions including proper eye, face, body wall, limb and neural tube development. They also suppress a number of genes including MCAM/MUC18, C/EBP alpha and MYC. AP-2-alpha is the only AP-2 protein required for early morphogenesis of the lens vesicle. Together with the CITED2 coactivator, stimulates the PITX2 P1 promoter transcription activation. Associates with chromatin to the PITX2 P1 promoter region.
Cellular Location Nucleus.
Citations (0)
citation

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References

1.Mol Hum Reprod. 2011 Nov;17(11):702-9. 2.Breast Cancer Res. 2011 Mar 4;13(2):R23.

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$ 385.00
Cat# AO2053a
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