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ERCC1 Antibody

Purified Mouse Monoclonal Antibody

     
  • E - ERCC1 Antibody AO2044a

    Figure 1:Black line: Control Antigen (100 ng);
    Purple line: Antigen(10ng);
    Blue line: Antigen (50 ng);
    Red line: Antigen (100 ng);

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  • WB - ERCC1 Antibody AO2044a

    Figure 2:Western blot analysis using ERCC1 mAb against human ERCC1 (AA: 151-297) recombinant protein. (Expected MW is 42.5 kDa)

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  • WB - ERCC1 Antibody AO2044a

    Figure 3:Western blot analysis using ERCC1 mAb against HEK293 (1) and ERCC1 (AA: 151-297)-hIgGFc transfected HEK293 (2) cell lysate.

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  • IF - ERCC1 Antibody AO2044a

    Figure 4:Immunofluorescence analysis of Hela cells using ERCC1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

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  • FC - ERCC1 Antibody AO2044a

    Figure 5:Flow cytometric analysis of A549 cells using ERCC1 mouse mAb (green) and negative control (red).

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  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, FC, ICC, E
Primary Accession P07992
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 1E5B3
Isotype IgG1
Calculated MW 32.6kDa
Description The product of this gene functions in the nucleotide excision repair pathway, and is required for the repair of DNA lesions such as those induced by UV light or formed by electrophilic compounds including cisplatin. The encoded protein forms a heterodimer with the XPF endonuclease (also known as ERCC4), and the heterodimeric endonuclease catalyzes the 5' incision in the process of excising the DNA lesion. The heterodimeric endonuclease is also involved in recombinational DNA repair and in the repair of inter-strand crosslinks. Mutations in this gene result in cerebrooculofacioskeletal syndrome, and polymorphisms that alter expression of this gene may play a role in carcinogenesis. Multiple transcript variants encoding different isoforms have been found for this gene. The last exon of this gene overlaps with the CD3e molecule, epsilon associated protein gene on the opposite strand. [provided by RefSeq, Oct 2009]
Immunogen Purified recombinant fragment of human ERCC1 (AA: 151-297) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide
Additional Information
Gene ID 2067
Other Names DNA excision repair protein ERCC-1, ERCC1
Dilution E~~1/10000
WB~~1/500 - 1/2000
IF~~1/200 - 1/1000
FC~~1/200 - 1/400
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsERCC1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ERCC1
Function [Isoform 1]: Non-catalytic component of a structure-specific DNA repair endonuclease responsible for the 5'-incision during DNA repair. Responsible, in conjunction with SLX4, for the first step in the repair of interstrand cross-links (ICL). Participates in the processing of anaphase bridge-generating DNA structures, which consist in incompletely processed DNA lesions arising during S or G2 phase, and can result in cytokinesis failure. Also required for homology-directed repair (HDR) of DNA double-strand breaks, in conjunction with SLX4.
Cellular Location [Isoform 1]: Nucleus [Isoform 3]: Nucleus
Research Areas
Citations (0)
citation

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References

Lung Cancer. 2013 Nov;82(2):324-9.Cancer. 2013 Sep 1;119(17):3242-50.

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$ 385.00
Cat# AO2044a
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