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MGMT Antibody
Purified Mouse Monoclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, E |
|---|---|
| Primary Accession | P16455 |
| Reactivity | Human |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone Names | 1A11A8 |
| Isotype | IgG1 |
| Calculated MW | 21.6kDa |
| Description | MGMT involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA.Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated |
| Immunogen | Purified recombinant fragment of human MGMT (AA: 32-210) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
| Gene ID | 4255 |
|---|---|
| Other Names | Methylated-DNA--protein-cysteine methyltransferase, 2.1.1.63, 6-O-methylguanine-DNA methyltransferase, MGMT, O-6-methylguanine-DNA-alkyltransferase, MGMT |
| Dilution | E~~1/10000 WB~~1/500 - 1/2000 |
| Storage | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | MGMT Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | MGMT |
|---|---|
| Function | Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) and O4-methylthymine (O4-MeT) in DNA. Repairs the methylated nucleobase in DNA by stoichiometrically transferring the methyl group to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated. |
| Cellular Location | Nucleus. |
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Provided below are standard protocols that you may find useful for product applications.
References
Folia Neuropathol. 2013;51(4):275-82.Fam Cancer. 2013 Sep;12(3):449-58.
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