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SELL Antibody

Purified Mouse Monoclonal Antibody

     
  • E - SELL Antibody AO2038a

    Figure 1:Black line: Control Antigen (100 ng);
    Purple line: Antigen(10ng);
    Blue line: Antigen (50 ng);
    Red line: Antigen (100 ng);

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  • WB - SELL Antibody AO2038a

    Figure 2:Western blot analysis using SELL mAb against human SELL (AA: 83-186) recombinant protein. (Expected MW is 37.4 kDa)

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  • WB - SELL Antibody AO2038a

    Figure 3:Western blot analysis using SELL mAb against HEK293 (1) and SELL (AA:83-186)-hIgGFc transfected HEK293 (2) cell lysate.

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  • IF - SELL Antibody AO2038a

    Figure 4:Immunofluorescence analysis of Hela cells using SELL mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

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  • IF - SELL Antibody AO2038a

    Figure 5:Immunofluorescence analysis of MCF-7 cells using SELL mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, ICC, E
Primary Accession P14151
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 8C8B7
Isotype IgG1
Calculated MW 42.2kDa
Description This gene encodes a cell surface adhesion molecule that belongs to a family of adhesion/homing receptors. The encoded protein contains a C-type lectin-like domain, a calcium-binding epidermal growth factor-like domain, and two short complement-like repeats. The gene product is required for binding and subsequent rolling of leucocytes on endothelial cells, facilitating their migration into secondary lymphoid organs and inflammation sites. Single-nucleotide polymorphisms in this gene have been associated with various diseases including immunoglobulin A nephropathy. Alternatively spliced transcript variants have been found for this gene.
Immunogen Purified recombinant fragment of human SELL (AA: 83-186) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide
Additional Information
Gene ID 6402
Other Names L-selectin, CD62 antigen-like family member L, Leukocyte adhesion molecule 1, LAM-1, Leukocyte surface antigen Leu-8, Leukocyte-endothelial cell adhesion molecule 1, LECAM1, Lymph node homing receptor, TQ1, gp90-MEL, CD62L, SELL, LNHR, LYAM1
Dilution E~~1/10000
WB~~1/500 - 1/2000
IF~~1/200 - 1/1000
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsSELL Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name SELL
Synonyms LNHR, LYAM1
Function Calcium-dependent lectin that mediates cell adhesion by binding to glycoproteins on neighboring cells (PubMed:12403782, PubMed:28011641, PubMed:28489325). Mediates the adherence of lymphocytes to endothelial cells of high endothelial venules in peripheral lymph nodes. Promotes initial tethering and rolling of leukocytes in endothelia (PubMed:12403782, PubMed:28011641).
Cellular Location Cell membrane; Single-pass type I membrane protein
Tissue Location Expressed in B-cell lines and T-lymphocytes.
Research Areas
Citations (0)
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References

PLoS One. 2012;7(9):e44814.Eur J Cell Biol. 2012 Apr;91(4):257-64.

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$ 385.00
Cat# AO2038a
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