ADD1 Antibody
Purified Mouse Monoclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, IHC, E |
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Primary Accession | P35611 |
Reactivity | Human |
Host | Mouse |
Clonality | Monoclonal |
Clone Names | 5D4H1 |
Isotype | IgG1 |
Calculated MW | 81kDa |
Description | Adducins are a family of cytoskeleton proteins encoded by three genes (alpha, beta, gamma). Adducin is a heterodimeric protein that consists of related subunits, which are produced from distinct genes but share a similar structure. Alpha- and beta-adducin include a protease-resistant N-terminal region and a protease-sensitive, hydrophilic C-terminal region. Alpha- and gamma-adducins are ubiquitously expressed. In contrast, beta-adducin is expressed at high levels in brain and hematopoietic tissues. Adducin binds with high affinity to Ca(2+)/calmodulin and is a substrate for protein kinases A and C. Alternative splicing results in multiple variants encoding distinct isoforms; however, not all variants have been fully described. |
Immunogen | Purified recombinant fragment of human ADD1 (AA: 1-193) expressed in E. Coli. |
Formulation | Purified antibody in PBS with 0.05% sodium azide |
Gene ID | 118 |
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Other Names | Alpha-adducin, Erythrocyte adducin subunit alpha, ADD1, ADDA |
Dilution | E~~1/10000 WB~~1/500 - 1/2000 IHC~~1/200 - 1/1000 |
Storage | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Precautions | ADD1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | ADD1 |
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Synonyms | ADDA |
Function | Membrane-cytoskeleton-associated protein that promotes the assembly of the spectrin-actin network. Binds to calmodulin. |
Cellular Location | Cytoplasm, cytoskeleton. Cell membrane; Peripheral membrane protein; Cytoplasmic side |
Tissue Location | Expressed in all tissues. Found in much higher levels in reticulocytes than the beta subunit |
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Provided below are standard protocols that you may find useful for product applications.
References
EMBO J. 2012 Mar 21;31(6):1453-66.Traffic. 2011 Oct;12(10):1327-40.
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