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WTAP Antibody
Purified Mouse Monoclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IHC, FC, E |
|---|---|
| Primary Accession | Q15007 |
| Reactivity | Human |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone Names | 6B6B8 |
| Isotype | IgG1 |
| Calculated MW | 44.2kDa |
| Description | The Wilms tumor suppressor gene WT1 appears to play a role in both transcriptional and posttranscriptional regulation of certain cellular genes. This gene encodes a WT1-associating protein, which is a ubiquitously expressed nuclear protein. Like WT1 protein, this protein is localized throughout the nucleoplasm as well as in speckles and partially colocalizes with splicing factors. Alternative splicing of this gene results in several transcript variants encoding three different isoforms. |
| Immunogen | Purified recombinant fragment of human WTAP (AA: 91-201) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
| Gene ID | 9589 |
|---|---|
| Other Names | Pre-mRNA-splicing regulator WTAP, Female-lethal(2)D homolog, hFL(2)D, WT1-associated protein, Wilms tumor 1-associating protein, WTAP, KIAA0105 |
| Dilution | E~~1/10000 WB~~1/500 - 1/2000 FC~~1/200 - 1/400 IHC~~1/200 - 1/1000 |
| Storage | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | WTAP Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | WTAP {ECO:0000303|PubMed:11001926, ECO:0000312|HGNC:HGNC:16846} |
|---|---|
| Function | Associated component of the WMM complex, a complex that mediates N6-methyladenosine (m6A) methylation of RNAs, a modification that plays a role in the efficiency of mRNA splicing and RNA processing (PubMed:29507755). Required for accumulation of METTL3 and METTL14 to nuclear speckle (PubMed:24316715, PubMed:24407421, PubMed:24981863). Acts as a mRNA splicing regulator (PubMed:12444081). Regulates G2/M cell-cycle transition by binding to the 3' UTR of CCNA2, which enhances its stability (PubMed:17088532). Impairs WT1 DNA-binding ability and inhibits expression of WT1 target genes (PubMed:17095724). |
| Cellular Location | Nucleus speckle. Nucleus, nucleoplasm. Cytoplasm {ECO:0000250|UniProtKB:Q9ER69}. Note=Mainly nuclear with some fraction located in the cytoplasm. ZC3H13 is required to anchor component of the MACOM subcomplex, such as VIRMA, in the nucleus {ECO:0000250|UniProtKB:Q9ER69} |
| Tissue Location | Ubiquitously expressed. |
Research Areas
Citations (0)
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info@abcepta.com, and receive a free "I Love Antibodies" mug.
Application Protocols
Provided below are standard protocols that you may find useful for product applications.
References
J Gastroenterol. 2013 Nov;48(11):1271-82.Cancer Sci. 2012 Dec;103(12):2102-9.
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$ 385.00
Cat# AO2021a
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