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THY1 Antibody

Purified Mouse Monoclonal Antibody

     
  • E - THY1 Antibody AO1848a

    Black line: Control Antigen (100 ng);
    Purple line: Antigen(10ng);
    Blue line: Antigen (50 ng);
    Red line: Antigen (100 ng);

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  • WB - THY1 Antibody AO1848a
    Figure 1: Western blot analysis using THY1 mAb against human THY1 recombinant protein. (Expected MW is 38.5 kDa)
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  • WB - THY1 Antibody AO1848a
    Figure 2: Western blot analysis using THY1 mAb against HEK293 (1) and THY1 (AA: 17-132)-hIgGFc transfected HEK293 (2) cell lysate.
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  • WB - THY1 Antibody AO1848a
    Figure 3: Western blot analysis using THY1 mouse mAb against T47D (1), HepG2 (2) and PC-12 (3) cell lysate.
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  • IF - THY1 Antibody AO1848a
    Figure 4: Immunofluorescence analysis of Hela cells using THY1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
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  • IHC - THY1 Antibody AO1848a
    Figure 5: Immunohistochemical analysis of paraffin-embedded endometrial cancer tissues using THY1 mouse mAb with DAB staining.
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  • IHC - THY1 Antibody AO1848a
    Figure 6: Immunohistochemical analysis of paraffin-embedded cerebellum tissues using THY1 mouse mAb with DAB staining.
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  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IHC, ICC, E
Primary Accession P04216
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 7E1B11
Isotype IgG1
Calculated MW 18kDa
Description THY1 may play a role in cell-cell or cell-ligand interactions during synaptogenesis and other events in the brain
Immunogen Purified recombinant fragment of human THY1 (AA: 17-132) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide
Additional Information
Gene ID 7070
Other Names Thy-1 membrane glycoprotein, CDw90, Thy-1 antigen, CD90, THY1
Dilution E~~1/10000
WB~~1/500 - 1/2000
IF~~1/200 - 1/1000
IHC~~1/200 - 1/1000
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsTHY1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name THY1
Function May play a role in cell-cell or cell-ligand interactions during synaptogenesis and other events in the brain.
Cellular Location Cell membrane; Lipid-anchor, GPI- anchor
Citations (0)
citation

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Background

This gene encodes a zinc finger transcription factor. The encoded protein likely plays a role in transcriptional repression of interleukin 2. Mutations in this gene have been associated with posterior polymorphous corneal dystrophy-3 and late-onset Fuchs endothelial corneal dystrophy. Alternatively spliced transcript variants encoding different isoforms have been described. ; ;

References

1. Acta Histochem. 2011 Dec;113(8):833-8. 2. J Immunol. 2012 Feb 1;188(3):981-91.

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$ 385.00
Cat# AO1848a
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