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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   CLGN Antibody   

CLGN Antibody

Purified Mouse Monoclonal Antibody

     
  • E - CLGN Antibody AO1826a

    Black line: Control Antigen (100 ng);
    Purple line: Antigen(10ng);
    Blue line: Antigen (50 ng);
    Red line: Antigen (100 ng);

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  • WB - CLGN Antibody AO1826a
    Figure 1: Western blot analysis using CLGN mAb against human CLGN recombinant protein. (Expected MW is 43.5 kDa)
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  • WB - CLGN Antibody AO1826a
    Figure 2: Western blot analysis using CLGN mAb against HEK293 (1) and CLGN (AA: 249-405)-hIgGFc transfected HEK293 (2) cell lysate.
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  • WB - CLGN Antibody AO1826a
    Figure 3: Western blot analysis using CLGN mouse mAb against LNCAP cell lysate.
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  • IF - CLGN Antibody AO1826a
    Figure 4: Immunofluorescence analysis of Hela cells using CLGN mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
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  • IHC - CLGN Antibody AO1826a
    Figure 5: Immunohistochemical analysis of paraffin-embedded endometrial cancer tissues using CLGN mouse mAb with DAB staining.
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  • IHC - CLGN Antibody AO1826a
    Figure 6: Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using CLGN mouse mAb with DAB staining.
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  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IHC, ICC, E
Primary Accession O14967
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 1C8B6
Isotype IgG1
Calculated MW 70kDa
Description Calmegin is a testis-specific endoplasmic reticulum chaperone protein. CLGN may play a role in spermatogeneisis and infertility.
Immunogen Purified recombinant fragment of human CLGN (AA: 249-405) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide
Additional Information
Gene ID 1047
Other Names Calmegin, CLGN
Dilution E~~1/10000
WB~~1/500 - 1/2000
IF~~1/200 - 1/1000
IHC~~1/200 - 1/1000
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCLGN Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CLGN
Function Functions during spermatogenesis as a chaperone for a range of client proteins that are important for sperm adhesion onto the egg zona pellucida and for subsequent penetration of the zona pellucida. Required for normal sperm migration from the uterus into the oviduct. Required for normal male fertility. Binds calcium ions (By similarity).
Cellular Location Endoplasmic reticulum membrane; Single-pass type I membrane protein
Tissue Location Detected in testis (at protein level). Detected in testis.
Research Areas
Citations (0)
citation

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Background

Calmegin is a testis-specific endoplasmic reticulum chaperone protein. CLGN may play a role in spermatogeneisis and infertility. ; ;

References

1. Arch Histol Cytol. 1999 Aug;62(3):283-93. 2. Dev Biol. 2001 Dec 1;240(1):254-61.

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$ 385.00
Cat# AO1826a
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