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CD24 Antibody

Purified Mouse Monoclonal Antibody

     
  • E - CD24 Antibody AO1822a

    Black line: Control Antigen (100 ng);
    Purple line: Antigen(10ng);
    Blue line: Antigen (50 ng);
    Red line: Antigen (100 ng);

    detail
  • WB - CD24 Antibody AO1822a
    Figure 1: Western blot analysis using CD24 mAb against human CD24 recombinant protein. (Expected MW is 32.1 kDa)
    detail
  • WB - CD24 Antibody AO1822a
    Figure 2: Western blot analysis using CD24 mAb against HEK293 (1) and CD24 (AA: 15-80)-hIgGFc transfected HEK293 (2) cell lysate.
    detail
  • FC - CD24 Antibody AO1822a
    Figure 3: Flow cytometric analysis of Hela cells using CD24 mouse mAb (green) and negative control (red).
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  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, FC, E
Primary Accession P25063
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 4F4E10
Isotype IgG1
Calculated MW 8kDa
Description This gene encodes a sialoglycoprotein that is expressed on mature granulocytes and in many B cells. The encoded protein is anchored via a glycosyl phosphatidylinositol (GPI) link to the cell surface.
Immunogen Purified recombinant fragment of human CD24 (AA: 15-80) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide
Additional Information
Gene ID 100133941
Other Names Signal transducer CD24, Small cell lung carcinoma cluster 4 antigen, CD24, CD24, CD24A
Dilution E~~1/10000
WB~~1/500 - 1/2000
FC~~1/200 - 1/400
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCD24 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CD24
Synonyms CD24A
Function May have a pivotal role in cell differentiation of different cell types. Signaling could be triggered by the binding of a lectin- like ligand to the CD24 carbohydrates, and transduced by the release of second messengers derived from the GPI-anchor. Modulates B-cell activation responses. Promotes AG-dependent proliferation of B-cells, and prevents their terminal differentiation into antibody-forming cells (PubMed:11313396). In association with SIGLEC10 may be involved in the selective suppression of the immune response to danger-associated molecular patterns (DAMPs) such as HMGB1, HSP70 and HSP90. Plays a role in the control of autoimmunity (By similarity).
Cellular Location Cell membrane; Lipid-anchor, GPI-anchor.
Tissue Location B-cells. Expressed in a number of B-cell lines including P32/ISH and Namalwa. Expressed in erythroleukemia cell and small cell lung carcinoma cell lines. Also expressed on the surface of T-cells.
Research Areas
Citations (0)
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Background

This gene encodes a sialoglycoprotein that is expressed on mature granulocytes and in many B cells. The encoded protein is anchored via a glycosyl phosphatidylinositol (GPI) link to the cell surface. ; ;

References

1. Cancer Res. 2012 Nov 1;72(21):5600-12. 2. Stem Cell Rev. 2012 Sep;8(3):779-91.

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$ 385.00
Cat# AO1822a
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