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TUBB2A Antibody

Purified Mouse Monoclonal Antibody

     
  • E - TUBB2A Antibody AO1798a

    Black line: Control Antigen (100 ng);
    Purple line: Antigen(10ng);
    Blue line: Antigen (50 ng);
    Red line: Antigen (100 ng);

    detail
  • WB - TUBB2A Antibody AO1798a
    Figure 1: Western blot analysis using TUBB2A mAb against human TUBB2A recombinant protein. (Expected MW is 43.2 kDa)
    detail
  • WB - TUBB2A Antibody AO1798a
    Figure 2: Western blot analysis using TUBB2A mAb against HEK293 (1) and TUBB2A (AA: 25-187)-hIgGFc transfected HEK293 (2) cell lysate.
    detail
  • WB - TUBB2A Antibody AO1798a
    Figure 3: Western blot analysis using TUBB2A mouse mAb against HeLa (1), A549 (2), HEK293 (3), Jurkat (4) and PC-12 (5) cell lysate.
    detail
  • FC - TUBB2A Antibody AO1798a
    Figure 4: Flow cytometric analysis of Hela cells using TUBB2A mouse mAb (green) and negative control (red).
    detail
  • SPECIFICATION
  • CITATIONS
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  • BACKGROUND
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, FC, E
Primary Accession Q13885
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 6A10B2
Isotype IgG1
Calculated MW 50kDa
Description Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain.
Immunogen Purified recombinant fragment of human TUBB2A (AA: 25-187) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide
Additional Information
Gene ID 7280
Other Names Tubulin beta-2A chain, Tubulin beta class IIa, TUBB2A, TUBB2
Dilution E~~1/10000
WB~~1/500 - 1/2000
FC~~1/200 - 1/400
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsTUBB2A Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name TUBB2A
Synonyms TUBB2
Function Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin.
Cellular Location Cytoplasm, cytoskeleton.
Tissue Location High expression in brain, where it represents 30% of all beta-tubulins.
Research Areas
Citations (0)
citation

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Background

The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the basal layer of the epidermis with family member KRT14. Mutations in these genes have been associated with a complex of diseases termed epidermolysis bullosa simplex. The type II cytokeratins are clustered in a region of chromosome 12q12-q13.

References

1. Clin Cancer Res. 2012 Aug 15;18(16):4441-8. 2. Pathol Int. 2012 Apr;62(4):287-90.

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$ 385.00
Cat# AO1798a
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Availability: 7-10 days
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