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T Antibody

Purified Mouse Monoclonal Antibody

     
  • E - T Antibody AO1777a

    Black line: Control Antigen (100 ng);
    Purple line: Antigen(10ng);
    Blue line: Antigen (50 ng);
    Red line: Antigen (100 ng);

    detail
  • WB - T Antibody AO1777a
    Figure 1: Western blot analysis using T mAb against human T recombinant protein. (Expected MW is 31.2 kDa)
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  • WB - T Antibody AO1777a
    Figure 2: Western blot analysis using T mAb against HEK293 (1) and T (AA: 257-309)-hIgGFc transfected HEK293 (2) cell lysate.
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  • WB - T Antibody AO1777a
    Figure 3: Western blot analysis using T mouse mAb against Raji (1), and Jurkat (2) cell lysate.
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  • FC - T Antibody AO1777a
    Figure 4: Flow cytometric analysis of HeLa cells using T mouse mAb (green) and negative control (red).
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  • IHC - T Antibody AO1777a
    Figure 5: Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using T mouse mAb with DAB staining.
    detail
  • IHC - T Antibody AO1777a
    Figure 6: Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using T mouse mAb with DAB staining.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IHC, FC, E
Primary Accession O15178
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 1H9A2
Isotype IgG1
Calculated MW 47.4kDa
Description The protein encoded by this gene is an embryonic nuclear transcription factor that binds to a specific DNA element, the palindromic T-site. It binds through a region in its N-terminus, called the T-box, and effects transcription of genes required for mesoderm formation and differentiation. The protein is localized to notochord-derived cells.
Immunogen Purified recombinant fragment of human T (AA: 257-309 ) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide
Additional Information
Gene ID 6862
Other Names Brachyury protein, Protein T, T
Dilution E~~1/10000
WB~~1/500 - 1/2000
FC~~1/200 - 1/400
IHC~~1/200 - 1/1000
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsT Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name TBXT (HGNC:11515)
Synonyms T
Function Involved in the transcriptional regulation of genes required for mesoderm formation and differentiation. Binds to a palindromic T site 5'-TTCACACCTAGGTGTGAA-3' DNA sequence and activates gene transcription when bound to such a site.
Cellular Location Nucleus
Tissue Location Detected in testis, but not in other, normal tissues. Detected in lung tumors (at protein level)
Research Areas
Citations (0)
citation

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References

1.Am J Surg Pathol. 2008 Apr;32(4):572-80. 2.J Clin Neurosci. 2011 Jan;18(1):96-9.

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$ 385.00
Cat# AO1777a
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