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SPI1 Antibody

Purified Mouse Monoclonal Antibody

     
  • E - SPI1 Antibody AO1739a

    Black line: Control Antigen (100 ng);
    Purple line: Antigen(10ng);
    Blue line: Antigen (50 ng);
    Red line: Antigen (100 ng);

    detail
  • WB - SPI1 Antibody AO1739a
    Figure 1: Western blot analysis using SPI1 mAb against human SPI1 recombinant protein. (Expected MW is 46.9 kDa)
    detail
  • WB - SPI1 Antibody AO1739a
    Figure 2: Western blot analysis using SPI1 mAb against HEK293 (1) and SPI1 (AA: 124-271)-hIgGFc transfected HEK293 (2) cell lysate.
    detail
  • FC - SPI1 Antibody AO1739a
    Figure 3: Flow cytometric analysis of HeLa cells using SPI1 mouse mAb (green) and negative control (red).
    detail
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, FC, E
Primary Accession P17947
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 5C7
Isotype IgG1
Calculated MW 42kDa
Description This gene encodes an ETS-domain transcription factor that activates gene expression during myeloid and B-lymphoid cell development. The nuclear protein binds to a purine-rich sequence known as the PU-box found near the promoters of target genes, and regulates their expression in coordination with other transcription factors and cofactors. The protein can also regulate alternative splicing of target genes. Multiple transcript variants encoding different isoforms have been found for this gene.
Immunogen Purified recombinant fragment of human SPI1 (AA: 124-271) expressed in E. Coli.

Formulation Ascitic fluid containing 0.03% sodium azide.


Additional Information
Gene ID 6688
Other Names Transcription factor PU.1, 31 kDa-transforming protein, SPI1
Dilution E~~1/10000
WB~~1/500 - 1/2000
FC~~1/200 - 1/400
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsSPI1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name SPI1
Function Pioneer transcription factor, which controls hematopoietic cell fate by decompacting stem cell heterochromatin and allowing other transcription factors to enter otherwise inaccessible genomic sites. Once in open chromatin, can directly control gene expression by binding genetic regulatory elements and can also more broadly influence transcription by recruiting transcription factors, such as interferon regulatory factors (IRFs), to otherwise inaccessible genomic regions (PubMed:23658224, PubMed:33951726). Transcriptionally activates genes important for myeloid and lymphoid lineages, such as CSF1R (By similarity). Transcriptional activation from certain promoters, possibly containing low affinity binding sites, is achieved cooperatively with other transcription factors. FCER1A transactivation is achieved in cooperation with GATA1 (By similarity). May be particularly important for the pro- to pre-B cell transition (PubMed:33951726). Binds (via the ETS domain) onto the purine-rich DNA core sequence 5'-GAGGAA-3', also known as the PU-box (PubMed:33951726). In vitro can bind RNA and interfere with pre-mRNA splicing (By similarity).
Cellular Location Nucleus {ECO:0000255|PROSITE-ProRule:PRU00237, ECO:0000269|PubMed:33951726}
Tissue Location In the bone marrow, concentrated in hematopoietic stem cell, lymphoid progenitor, myeloid lineage (granulocyte macrophage progenitors, classical dendritic cells, monocytes) and B-cell clusters Among B-cells, predominantly expressed in pre-B1 cells (PubMed:33951726). Expressed in germinal center B-cells (PubMed:23166356).
Research Areas
Citations (0)
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References

1.J Biol Chem. 2011 Jan 21;286(3):1675-82.2.Leuk Res. 2010 Dec;34(12):1636-46.

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$ 385.00
Cat# AO1739a
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