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ARHGDIA Antibody

Purified Mouse Monoclonal Antibody

     
  • E - ARHGDIA Antibody AO1737a

    Black line: Control Antigen (100 ng);
    Purple line: Antigen(10ng);
    Blue line: Antigen (50 ng);
    Red line: Antigen (100 ng);

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  • WB - ARHGDIA Antibody AO1737a
    Figure 1: Western blot analysis using ARHGDIA mAb against human ARHGDIA recombinant protein. (Expected MW is 48.7 kDa)
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  • WB - ARHGDIA Antibody AO1737a
    Figure 2: Western blot analysis using ARHGDIA mouse mAb against Jurkat (1), HeLa (2), NIH3T3 (3), C6 (4), K562 (5), and COS7 (6) cell lysate.
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  • IF - ARHGDIA Antibody AO1737a
    Figure 3: Immunofluorescence analysis of HepG2 cells using ARHGDIA mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.
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  • FC - ARHGDIA Antibody AO1737a
    Figure 4: Flow cytometric analysis of HeLa cells using ARHGDIA mouse mAb (green) and negative control (red).
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  • SPECIFICATION
  • CITATIONS
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  • BACKGROUND
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, FC, ICC, E
Primary Accession P52565
Reactivity Human, Mouse, Monkey
Host Mouse
Clonality Monoclonal
Clone Names 2G3
Isotype IgG1
Calculated MW 26kDa
Description Aplysia Ras-related homologs (ARHs), also called Rho genes, belong to the RAS gene superfamily encoding small guanine nucleotide exchange (GTP/GDP) factors. The ARH proteins may be kept in the inactive, GDP-bound state by interaction with GDP dissociation inhibitors, such as ARHGDIA
Immunogen Purified recombinant fragment of human ARHGDIA (AA: FULL(1-204)) expressed in E. Coli.

Formulation Purified antibody in PBS with 0.05% sodium azide
Additional Information
Gene ID 396
Other Names Rho GDP-dissociation inhibitor 1, Rho GDI 1, Rho-GDI alpha, ARHGDIA, GDIA1
Dilution E~~1/10000
WB~~1/500 - 1/2000
IF~~1/200 - 1/1000
FC~~1/200 - 1/400
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsARHGDIA Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ARHGDIA
Synonyms GDIA1
Function Controls Rho proteins homeostasis. Regulates the GDP/GTP exchange reaction of the Rho proteins by inhibiting the dissociation of GDP from them, and the subsequent binding of GTP to them. Retains Rho proteins such as CDC42, RAC1 and RHOA in an inactive cytosolic pool, regulating their stability and protecting them from degradation. Actively involved in the recycling and distribution of activated Rho GTPases in the cell, mediates extraction from membranes of both inactive and activated molecules due its exceptionally high affinity for prenylated forms. Through the modulation of Rho proteins, may play a role in cell motility regulation. In glioma cells, inhibits cell migration and invasion by mediating the signals of SEMA5A and PLXNB3 that lead to inactivation of RAC1.
Cellular Location Cytoplasm.
Research Areas
Citations (0)
citation

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References

1.Nat Cell Biol. 2010 May;12(5):477-83. 2.Int J Oncol. 2010 Feb;36(2):379-86.

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$ 385.00
Cat# AO1737a
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