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ASS1 Antibody

Purified Mouse Monoclonal Antibody

     
  • E - ASS1 Antibody AO1671a

    Black line: Control Antigen (100 ng);
    Purple line: Antigen(10ng);
    Blue line: Antigen (50 ng);
    Red line: Antigen (100 ng);

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  • WB - ASS1 Antibody AO1671a
    Figure 1: Western blot analysis using ASS1 mAb against human ASS1 (AA: 40-236) recombinant protein. (Expected MW is 47 kDa)
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  • WB - ASS1 Antibody AO1671a
    Figure 2: Western blot analysis using ASS1 mouse mAb against A431 (1), RAJI (2), MOLT4 (3), Jurkat (4), A549 (5), NIH/3T3 (6), PC-12 (7) and Cos7 (8) cell lysate.
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  • IHC - ASS1 Antibody AO1671a
    Figure 3: Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using ASS1 mouse mAb with DAB staining.
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  • IHC - ASS1 Antibody AO1671a
    Figure 4: Immunohistochemical analysis of paraffin-embedded colon cancer tissues using ASS1 mouse mAb with DAB staining.
    detail
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IHC, E
Primary Accession P00966
Reactivity Human, Mouse, Monkey
Host Mouse
Clonality Monoclonal
Clone Names 2C10
Isotype IgG1
Calculated MW 47kDa
Description The protein encoded by this gene catalyzes the penultimate step of the arginine biosynthetic pathway. There are approximately 10 to 14 copies of this gene including the pseudogenes scattered across the human genome, among which the one located on chromosome 9 appears to be the only functional gene for argininosuccinate synthetase. Mutations in the chromosome 9 copy of ASS cause citrullinemia. Two transcript variants encoding the same protein have been found for this gene.
Immunogen Purified recombinant fragment of human ASS1 expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide
Additional Information
Gene ID 445
Other Names Argininosuccinate synthase, 6.3.4.5, Citrulline--aspartate ligase, ASS1, ASS
Dilution E~~1/10000
WB~~1/500 - 1/2000
IHC~~1/200 - 1/1000
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsASS1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ASS1 (HGNC:758)
Function One of the enzymes of the urea cycle, the metabolic pathway transforming neurotoxic amonia produced by protein catabolism into inocuous urea in the liver of ureotelic animals. Catalyzes the formation of arginosuccinate from aspartate, citrulline and ATP and together with ASL it is responsible for the biosynthesis of arginine in most body tissues.
Cellular Location Cytoplasm, cytosol
Tissue Location Expressed in adult liver.
Research Areas
Citations (0)
citation

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References

1. Int J Cancer. 2009 Sep 15;125(6):1454-63. 2. Clin Biochem. 2009 Jul;42(10-11):1166-8.

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$ 385.00
Cat# AO1671a
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