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OTX2 Antibody

Purified Mouse Monoclonal Antibody

     
  • E - OTX2 Antibody AO1568a

    Red: Control Antigen (100ng);
    Purple: Antigen (10ng);
    Green: Antigen (50ng);
    Blue: Antigen (100ng);

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  • WB - OTX2 Antibody AO1568a
    Figure 1: Western blot analysis using OTX2 mouse mAb against HepG2 (1), Jurkat (2), and NTERA-2 (3) cell lysate.
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  • WB - OTX2 Antibody AO1568a
    Figure 2: Western blot analysis using OTX2 mAb against human OTX2 (AA: 40-297) recombinant protein. (Expected MW is 65 kDa)
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  • IHC - OTX2 Antibody AO1568a
    Figure 3: Immunohistochemical analysis of paraffin-embedded colon tissues (left) and colon cancer tissues (right) using OTX2 mouse mAb with DAB staining.
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  • IHC - OTX2 Antibody AO1568a
    Figure 4: Immunohistochemical analysis of paraffin-embedded stomach tissues (left) and brain tissues (right) using OTX2 mouse mAb with DAB staining.
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  • IF - OTX2 Antibody AO1568a
    Figure 5: Immunofluorescence analysis of U251 cells using OTX2 mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IHC, ICC, E
Primary Accession P32243
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 1H12G8B2
Isotype IgG1
Calculated MW 32kDa
Description This gene encodes a member of the bicoid sub-family of homeodomain-containing transcription factors. The encoded protein acts as a transcription factor and may play a role in brain and sensory organ development. A similar protein in mice is required for proper forebrain development.Tissue specificity: Expressed in brain.
Immunogen Purified recombinant fragment of human OTX2 expressed in E. Coli.
Formulation Ascitic fluid containing 0.03% sodium azide.
Additional Information
Gene ID 5015
Other Names Homeobox protein OTX2, Orthodenticle homolog 2, OTX2
Dilution E~~1/10000
WB~~1/500 - 1/2000
IHC~~1/200 - 1/1000
IF~~1/200 - 1/1000
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsOTX2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name OTX2
Function Transcription factor probably involved in the development of the brain and the sense organs. Can bind to the bicoid/BCD target sequence (BTS): 5'-TCTAATCCC-3'.
Cellular Location Nucleus.
Research Areas
Citations (0)
citation

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References

1. Hum Mutat. 2008 Nov;29(11):E278-83. 2. Cancer Res. 2010 Jan 1;70(1):181-91.

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$ 385.00
Cat# AO1568a
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