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CD1A Antibody

Purified Mouse Monoclonal Antibody

     
  • E - CD1A Antibody AO1538a

    Red: Control Antigen (100ng);
    Purple: Antigen (10ng);
    Green: Antigen (50ng);
    Blue: Antigen (100ng);

    detail
  • WB - CD1A Antibody AO1538a
    Figure 1: Western blot analysis using CD1A mouse mAb against K562 (1), RAJI (2), and MOLT4 (3) cell lysate.
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  • IHC - CD1A Antibody AO1538a
    Figure 2: Immunohistochemical analysis of paraffin-embedded cervical cancer tissues (left) and colon cancer tissues (right) using CD1A mouse mAb with DAB staining.
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  • IHC - CD1A Antibody AO1538a
    Figure 3: Immunohistochemical analysis of paraffin-embedded brain tissues (left) and submaxillary tumor tissues (right) using CD1A mouse mAb with DAB staining.
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  • IF - CD1A Antibody AO1538a
    Figure 4: Immunofluorescence analysis of RAJI cells using CD1A mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.
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  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IHC, ICC, E
Primary Accession P06126
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 7A7
Isotype IgG1
Calculated MW 37kDa
Description CD1a is a non polymorphic MHC Class 1 related cell surface glycoprotein, expressed in association with Beta 2 microglobulin. CD1a is expressed by cortical thymocytes, Langerhan's cells and by interdigitating cells. CD1a is also expressed by some malignancies of T cell lineage and in histiocytosis X. Tissue specificity: Expressed on cortical thymocytes, epidermal Langerhans cells, dendritic cells, on certain T-cell leukemias, and in various other tissues.
Immunogen Purified recombinant fragment of human CD1A expressed in E. Coli.
Formulation Ascitic fluid containing 0.03% sodium azide.
Additional Information
Gene ID 909
Other Names T-cell surface glycoprotein CD1a, T-cell surface antigen T6/Leu-6, hTa1 thymocyte antigen, CD1a, CD1A
Dilution E~~1/10000
WB~~1/500 - 1/2000
IHC~~1/200 - 1/1000
IF~~1/200 - 1/1000
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCD1A Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CD1A
Function Antigen-presenting protein that binds self and non-self lipid and glycolipid antigens and presents them to T-cell receptors on natural killer T-cells.
Cellular Location Cell membrane; Single-pass type I membrane protein. Membrane raft; Single-pass type I membrane protein. Endosome membrane; Single- pass type I membrane protein. Note=Subject to intracellular trafficking between the cell membrane and endosomes (PubMed:11231314). Localizes to cell surface lipid rafts (PubMed:18178838).
Tissue Location Expressed on cortical thymocytes, epidermal Langerhans cells, dendritic cells, on certain T-cell leukemias, and in various other tissues.
Research Areas
Citations (0)
citation

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References

1. J Neuroimmunol. 2008 Dec 15;205(1-2):110-2. 2. Pathol Int. 2008 Mar;58(3):169-73.

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$ 385.00
Cat# AO1538a
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