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CDC25C Antibody

Purified Mouse Monoclonal Antibody

     
  • WB - CDC25C Antibody AO1375a
    Figure 1: Western blot analysis using anti-CDC25C mAb against Hela (1), K562 (2), PC-3 (3), HEK293 (4) and Raw264.7 (5) cell lysate.
    detail
  • IHC - CDC25C Antibody AO1375a
    Figure 2: Immunohistochemical analysis of paraffin-embedded human Testis tissues using anti-CDC25C mouse mAb
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  • FC - CDC25C Antibody AO1375a
    Figure 3: Flow cytometric analysis of PC-2 cells using CDC2 mouse mAb (green) and negative control (purple).
    detail
  • ICC - CDC25C Antibody AO1375a
    Figure 2: Immunofluorescence analysis of Hela cells using CDC2 mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
    detail
  • IF - CDC25C Antibody AO1375a
    Figure 2:Immunofluorescence analysis of Hela cells using CDC2 mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
    detail
  • SPECIFICATION
  • CITATIONS
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IHC, E
Primary Accession P30307
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 1F12
Isotype IgG1
Calculated MW 58kDa
Description Cdc25C is a tyrosine phosphatase and belongs to the Cdc25 phosphatase family. It has been highly conserved during evolution and it plays a key role in the regulation of cell division. It directs dephosphorylation of cyclin B-bound CDC2 and triggers entry into mitosis. It is also thought to suppress p53-induced growth arrest. Cdc25C is mainly expressed in G2 phase. Multiple alternatively spliced transcript variants of this gene have been described, however, the full-length nature of many of them is not known.
Immunogen Purified recombinant fragment of human CDC25C expressed in E. Coli.
Formulation Ascitic fluid containing 0.03% sodium azide.
Additional Information
Gene ID 995
Other Names M-phase inducer phosphatase 3, 3.1.3.48, Dual specificity phosphatase Cdc25C, CDC25C
Dilution WB~~1/500 - 1/2000
IHC~~1/200 - 1/1000
FC~~1:200~~400
ICC~~1:200~~1000
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCDC25C Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CDC25C
Function Functions as a dosage-dependent inducer in mitotic control. Tyrosine protein phosphatase required for progression of the cell cycle (PubMed:8119945). When phosphorylated, highly effective in activating G2 cells into prophase (PubMed:8119945). Directly dephosphorylates CDK1 and activates its kinase activity (PubMed:8119945).
Cellular Location Nucleus
Research Areas
Citations (0)
citation

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References

1. Cancer Cell. 2007 Mar;11(3):275-89. 2. Int J Biochem Cell Biol. 2007;39(9):1707-13. 3. Int J Cancer. 2010 May 1;126(9):2199-210.

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$ 325.00
Cat# AO1375a
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