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CDC25C Antibody
Purified Mouse Monoclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IHC, E |
|---|---|
| Primary Accession | P30307 |
| Reactivity | Human |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone Names | 1F12 |
| Isotype | IgG1 |
| Calculated MW | 58kDa |
| Description | Cdc25C is a tyrosine phosphatase and belongs to the Cdc25 phosphatase family. It has been highly conserved during evolution and it plays a key role in the regulation of cell division. It directs dephosphorylation of cyclin B-bound CDC2 and triggers entry into mitosis. It is also thought to suppress p53-induced growth arrest. Cdc25C is mainly expressed in G2 phase. Multiple alternatively spliced transcript variants of this gene have been described, however, the full-length nature of many of them is not known. |
| Immunogen | Purified recombinant fragment of human CDC25C expressed in E. Coli. |
| Formulation | Ascitic fluid containing 0.03% sodium azide. |
| Gene ID | 995 |
|---|---|
| Other Names | M-phase inducer phosphatase 3, 3.1.3.48, Dual specificity phosphatase Cdc25C, CDC25C |
| Dilution | WB~~1/500 - 1/2000 IHC~~1/200 - 1/1000 FC~~1:200~~400 ICC~~1:200~~1000 |
| Storage | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | CDC25C Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | CDC25C |
|---|---|
| Function | Functions as a dosage-dependent inducer in mitotic control. Tyrosine protein phosphatase required for progression of the cell cycle (PubMed:8119945). When phosphorylated, highly effective in activating G2 cells into prophase (PubMed:8119945). Directly dephosphorylates CDK1 and activates its kinase activity (PubMed:8119945). |
| Cellular Location | Nucleus |
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Provided below are standard protocols that you may find useful for product applications.
References
1. Cancer Cell. 2007 Mar;11(3):275-89. 2. Int J Biochem Cell Biol. 2007;39(9):1707-13. 3. Int J Cancer. 2010 May 1;126(9):2199-210.
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