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ETS1 Antibody

Purified Mouse Monoclonal Antibody

     
  • WB - ETS1 Antibody AO1371a
    Figure 1: Western blot analysis using ETS1 mouse mAb against Jurkat (1), HepG2 (2) and ETS1-hIgGFc transfected HEK293 (3) cell lysate.
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  • IHC - ETS1 Antibody AO1371a
    Figure 2: Immunohistochemical analysis of paraffin-embedded human Colon, Adipocytes tissues using anti-ETS1 mouse mAb
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  • IF - ETS1 Antibody AO1371a
    Figure 3: Immunofluorescence analysis of NIH/3T3 cells using anti-ETS1 mAb (green). Blue: DRAQ5 fluorescent DNA dye.
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  • FC - ETS1 Antibody AO1371a
    Figure 4: Flow cytometric analysis of Jurkat cells using anti-ETS1 mAb (green) and negative control (purple).
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  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IHC, FC, ICC, E
Primary Accession P14921
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 8A8
Isotype IgG1
Calculated MW 50.4kDa
Description The ETS1 is a transcription factor known to regulate expression of a number of genes involved in extracellular matrix remodeling. ETS1 is absent from normal gastric epithelium but is expressed in approximately 60% of gastric carcinomas and oral squamous cell carcinomas with a significant correlation to the tumor stage.
Immunogen Purified recombinant fragment of human ETS1 expressed in E. Coli.
Formulation Ascitic fluid containing 0.03% sodium azide.
Additional Information
Gene ID 2113
Other Names Protein C-ets-1, p54, ETS1, EWSR2
Dilution WB~~1/500 - 1/2000
IHC~~1/200 - 1/1000
IF~~1/200 - 1/1000
FC~~1/200 - 1/400
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsETS1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ETS1
Synonyms EWSR2
Function Transcription factor (PubMed:10698492, PubMed:11909962). Directly controls the expression of cytokine and chemokine genes in a wide variety of different cellular contexts (PubMed:20378371). May control the differentiation, survival and proliferation of lymphoid cells (PubMed:20378371). May also regulate angiogenesis through regulation of expression of genes controlling endothelial cell migration and invasion (PubMed:15247905, PubMed:15592518).
Cellular Location Nucleus. Cytoplasm Note=Delocalizes from nucleus to cytoplasm when coexpressed with isoform Ets-1 p27.
Tissue Location Highly expressed within lymphoid cells. Isoforms c- ETS-1A and Ets-1 p27 are both detected in all fetal tissues tested, but vary with tissue type in adult tissues. None is detected in brain or kidney.
Research Areas
Citations (0)
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References

1. DNA Cell Biol. 2005 Feb;24(2):126-32. 2. Mol Hum Reprod. 2006 Feb;12(2):71-6. 3. J Biol Chem. 2008 Jan 11;283(2):951-62.

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$ 325.00
Cat# AO1371a
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