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NT5E Antibody
Purified Mouse Monoclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| IHC, E |
|---|---|
| Primary Accession | P21589 |
| Reactivity | Human |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone Names | 1D7 |
| Isotype | IgG1 |
| Calculated MW | 70kDa |
| Description | 5'-nucleotidase, ecto (NT5E), also known as CD73 (Cluster of Differentiation 73). Ecto-5-prime-nucleotidase (5-prime-ribonucleotide phosphohydrolase; EC 3.1.3.5) catalyzes the conversion at neutral pH of purine 5-prime mononucleotides to nucleosides, the preferred substrate being AMP. The enzyme consists of a dimer of 2 identical 70-kD subunits bound by a glycosyl phosphatidyl inositol linkage to the external face of the plasma membrane. The enzyme is used as a marker of lymphocyte differentiation. Consequently, a deficiency of NT5 occurs in a variety of immunodeficiency diseases (e.g., see MIM 102700, MIM 300300). Other forms of 5-prime nucleotidase exist in the cytoplasm and lysosomes and can be distinguished from ecto-NT5 by their substrate affinities, requirement for divalent magnesium ion, activation by ATP, and inhibition by inorganic phosphate. |
| Immunogen | Purified recombinant fragment of NT5E expressed in E. Coli. |
| Formulation | Ascitic fluid containing 0.03% sodium azide. |
| Gene ID | 4907 |
|---|---|
| Other Names | 5'-nucleotidase, 5'-NT, 3.1.3.5, Ecto-5'-nucleotidase, CD73, NT5E, NT5, NTE |
| Dilution | IHC~~1/200 - 1/1000 WB~~1:500~~2000 |
| Storage | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | NT5E Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | NT5E |
|---|---|
| Synonyms | NT5, NTE |
| Function | Catalyzes the hydrolysis of nucleotide monophosphates, releasing inorganic phosphate and the corresponding nucleoside, with AMP being the preferred substrate (PubMed:21933152, PubMed:22997138, PubMed:23142347, PubMed:24887587, PubMed:34403084). Shows a preference for ribonucleotide monophosphates over their equivalent deoxyribose forms (PubMed:34403084). Other substrates include IMP, UMP, GMP, CMP, dAMP, dCMP, dTMP, NAD and NMN (PubMed:21933152, PubMed:22997138, PubMed:23142347, PubMed:24887587, PubMed:34403084). |
| Cellular Location | Cell membrane; Lipid-anchor, GPI-anchor |
Research Areas
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Application Protocols
Provided below are standard protocols that you may find useful for product applications.
References
1. Oncol Rep. 2007 Jun;17(6):1341-6. 2. Neurochem Int. 2003 Dec;43(7):621-8.
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$ 325.00
Cat# AO1306a
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