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CD18 Antibody

Purified Mouse Monoclonal Antibody

     
  • IF - CD18 Antibody AO1286a
    Figure 1: Confocal immunofluorescence analysis of HL60 cells using CD18 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.
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  • IF - CD18 Antibody AO1286a
    Figure 2: Confocal immunofluorescence analysis of BCBL-1 cells (left) and L1210 cells (right) using CD18 mouse mAb (red). Blue: DRAQ5 fluorescent DNA dye.
    detail
  • WB - CD18 Antibody AO1286a
    Figure 1: Western blot analysis using PAK2 mouse mAb against Hela (1), Jurkat (2), A549 (3), HEK293 (4) and K562 (5) cell lysate.
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  • IHC - CD18 Antibody AO1286a
    Figure 2: Immunohistochemical analysis of paraffin-embedded human lung cancer (left) and gastric cancer (right) using PAK2 mouse mAb with DAB staining.
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  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
ICC, E
Primary Accession P05107
Reactivity Human, Mouse
Host Mouse
Clonality Monoclonal
Clone Names 10E12
Isotype IgG2b
Calculated MW 85kDa
Description CD18, also known as ITGB2 (integrin beta chain beta 2). Integrins are integral cell-surface proteins composed of an alpha chain and a beta chain. A given chain may combine with multiple partners resulting in different integrins. For example, beta 2 combines with the alpha L chain to form the integrin LFA-1, and combines with the alpha M chain to form the integrin Mac-1. Integrins are known to participate in cell adhesion as well as cell-surface mediated signalling. CD18 is expressed by most leucocytes. Defects in this gene are the cause of leukocyte adhesion deficiency type I (LAD1). Two transcript variants encoding the same protein have been identified for this gene.
Immunogen Purified recombinant fragment of CD18 expressed in E. Coli.
Formulation Ascitic fluid containing 0.03% sodium azide.
Additional Information
Gene ID 3689
Other Names Integrin beta-2, Cell surface adhesion glycoproteins LFA-1/CR3/p150, 95 subunit beta, Complement receptor C3 subunit beta, CD18, ITGB2, CD18, MFI7
Dilution IF~~1/200 - 1/1000
WB~~1:500~~2000
IHC~~1:200~~1000
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCD18 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ITGB2
Synonyms CD18, MFI7
Function Integrin ITGAL/ITGB2 is a receptor for ICAM1, ICAM2, ICAM3 and ICAM4. Integrin ITGAL/ITGB2 is also a receptor for the secreted form of ubiquitin-like protein ISG15; the interaction is mediated by ITGAL (PubMed:29100055). Integrins ITGAM/ITGB2 and ITGAX/ITGB2 are receptors for the iC3b fragment of the third complement component and for fibrinogen. Integrin ITGAX/ITGB2 recognizes the sequence G-P-R in fibrinogen alpha-chain. Integrin ITGAM/ITGB2 recognizes P1 and P2 peptides of fibrinogen gamma chain. Integrin ITGAM/ITGB2 is also a receptor for factor X. Integrin ITGAD/ITGB2 is a receptor for ICAM3 and VCAM1. Contributes to natural killer cell cytotoxicity (PubMed:15356110). Involved in leukocyte adhesion and transmigration of leukocytes including T-cells and neutrophils (PubMed:11812992, PubMed:28807980). Triggers neutrophil transmigration during lung injury through PTK2B/PYK2-mediated activation (PubMed:18587400). Integrin ITGAL/ITGB2 in association with ICAM3, contributes to apoptotic neutrophil phagocytosis by macrophages (PubMed:23775590). In association with alpha subunit ITGAM/CD11b, required for CD177-PRTN3- mediated activation of TNF primed neutrophils (PubMed:21193407).
Cellular Location Cell membrane; Single-pass type I membrane protein. Membrane raft; Single-pass type I membrane protein
Tissue Location Leukocytes (PubMed:23775590). Expressed in neutrophils (at protein level) (PubMed:21193407, PubMed:28807980)
Research Areas
Citations (0)
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References

1. Microcirculation. 2008 Aug;15(6):555-67. 2. Mol Immunol. 2008 Feb;45(3):709-18. 3. J Biol Chem. 2007 Aug 17;282(33):24310-9.

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$ 325.00
Cat# AO1286a
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