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EPHB4 Antibody
Purified Mouse Monoclonal Antibody
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- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, ICC, E |
|---|---|
| Primary Accession | P54760 |
| Reactivity | Human |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone Names | 5B5 |
| Isotype | IgG1 |
| Calculated MW | 108kDa |
| Description | EphB4: EPH receptor B4, also known as Htk, Myk1, Tyro11. Entrez Protein: NP_004435. It is a member of the Eph receptor family, which bind the ephrin ligand family. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. Ephrin receptors make up the largest subgroup of the receptor tyrosine kinase (RTK) family. The EphB4 protein binds to ephrin-B2 and plays an essential role in vascular development. |
| Immunogen | Purified recombinant extracellular fragment of human EPHB4 fused with hIgGFc tag expressed in HEK293 cell line. |
| Formulation | Ascitic fluid containing 0.03% sodium azide. |
| Gene ID | 2050 |
|---|---|
| Other Names | Ephrin type-B receptor 4, 2.7.10.1, Hepatoma transmembrane kinase, Tyrosine-protein kinase TYRO11, EPHB4, HTK, MYK1, TYRO11 |
| Dilution | WB~~1/500 - 1/2000 IF~~1/200 - 1/1000 IHC~~1:200~~1000 |
| Storage | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | EPHB4 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | EPHB4 |
|---|---|
| Synonyms | HTK, MYK1, TYRO11 |
| Function | Receptor tyrosine kinase which binds promiscuously transmembrane ephrin-B family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Together with its cognate ligand/functional ligand EFNB2 it is involved in the regulation of cell adhesion and migration, and plays a central role in heart morphogenesis, angiogenesis and blood vessel remodeling and permeability. EPHB4-mediated forward signaling controls cellular repulsion and segregation from EFNB2-expressing cells. |
| Cellular Location | Cell membrane; Single-pass type I membrane protein |
| Tissue Location | Abundantly expressed in placenta but also detected in kidney, liver, lung, pancreas, skeletal muscle and heart. Expressed in primitive and myeloid, but not lymphoid, hematopoietic cells. Also observed in cell lines derived from liver, breast, colon, lung, melanocyte and cervix. |
Research Areas
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Application Protocols
Provided below are standard protocols that you may find useful for product applications.
References
1. Br J Cancer. 2007 Apr 10;96(7):1083-91. 2. Dig Liver Dis. 2007 Aug;39(8):725-32.
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$ 325.00
Cat# AO1275a
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