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NANOG Antibody

Purified Mouse Monoclonal Antibody

     
  • WB - NANOG Antibody AO1238a
    Figure 1: Western blot analysis using NANOG mouse mAb against NTERA-2 cell lysate (2).
    detail
  • IF - NANOG Antibody AO1238a
    Figure 2: Confocal immunofluorescence analysis of NTERA-2 cells (left) and HeLa cells (right) using Nanog mouse mAb (green). Red: Actin filaments have been labeled with DY-554 phalloidin.
    detail
  • IHC - NANOG Antibody AO1238a
    Figure 2: Immunohistochemical analysis of paraffin-embedded human Placenta tissues using PEG10 mouse mAb
    detail
  • SPECIFICATION
  • CITATIONS: 2
  • PROTOCOLS
  • BACKGROUND
  • detail
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, ICC, E
Primary Accession Q9H9S0
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 1E6C4
Isotype IgG1
Calculated MW 35kDa
Description NANOG: Nanog homeobox. Entrez Protein NP_079141. Nanog is a divergent homeodomain protein that directs pluripotency and differentiation of undifferentiated embryonic stem cells. Nanog mRNA is present in pluripotent mouse and human cell lines, and absent from differentiated cells. Human Nanog protein shares 52% overall amino acid identity with the mouse protein and 85% identity in the homeodomain. Human Nanog maps to gene locus 12p13.31, whereas mouse Nanog maps to gene loci 6 F2. Murine embryonic Nanog expression is detected in the inner cell mass of the blastocyst. High levels of human Nanog expression were detected by Northern analysis in the undifferentiated N-Tera embryonal carcinoma cell line.
Immunogen Purified recombinant fragment of NANOG (aa20-166) expressed in E. Coli.
Formulation Ascitic fluid containing 0.03% sodium azide.
Additional Information
Gene ID 79923
Other Names Homeobox protein NANOG, Homeobox transcription factor Nanog, hNanog, NANOG
Dilution WB~~1/500 - 1/2000
IF~~1/200 - 1/1000
IHC~~1:200~~1000
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsNANOG Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name NANOG
Function Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes. Acts as a transcriptional activator or repressor. Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]- 3'. Binds to the POU5F1/OCT4 promoter (PubMed:25825768). Able to autorepress its expression in differentiating (ES) cells: binds to its own promoter following interaction with ZNF281/ZFP281, leading to recruitment of the NuRD complex and subsequent repression of expression. When overexpressed, promotes cells to enter into S phase and proliferation.
Cellular Location Nucleus {ECO:0000255|PROSITE-ProRule:PRU00108, ECO:0000269|PubMed:15983365}
Tissue Location Expressed in testicular carcinoma and derived germ cell tumors (at protein level). Expressed in fetal gonads, ovary and testis. Also expressed in ovary teratocarcinoma cell line and testicular embryonic carcinoma. Not expressed in many somatic organs and oocytes.
Research Areas
Citations ( 0 )

References

1. Genes Cells. 2006 Sep;11(9):1115-23. 2. Mol Biol Cell. 2007 May;18(5):1543-53.

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$ 325.00
Cat# AO1238a
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