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MAP4K4 Antibody
Purified Mouse Monoclonal Antibody
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Application 
| WB, E |
|---|---|
| Primary Accession | O95819 |
| Reactivity | Human |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone Names | 4H9E7 |
| Isotype | IgG1 |
| Calculated MW | 142101 Da |
| Description | MAP4K4: mitogen-activated protein kinase kinase kinase kinase 4. The protein encoded by this gene is a member of the serine/threonine protein kinase family. This kinase has been shown to specifically activate MAPK8/JNK. The activation of MAPK8 by this kinase is found to be inhibited by the dominant-negative mutants of MAP3K7/TAK1, MAP2K4/MKK4, and MAP2K7/MKK7, which suggests that this kinase may function through the MAP3K7-MAP2K4-MAP2K7 kinase cascade, and mediate the TNF-alpha signaling pathway. Alternatively spliced transcript variants encoding different isoforms have been identified. |
| Immunogen | Purified recombinant fragment of MAP4K4 (aa400-500) expressed in E. Coli. |
| Formulation | Ascitic fluid containing 0.03% sodium azide. |
| Gene ID | 9448 |
|---|---|
| Other Names | Mitogen-activated protein kinase kinase kinase kinase 4, 2.7.11.1, HPK/GCK-like kinase HGK, MAPK/ERK kinase kinase kinase 4, MEK kinase kinase 4, MEKKK 4, Nck-interacting kinase, MAP4K4, HGK, KIAA0687, NIK |
| Dilution | WB~~1/500 - 1/2000 IHC~~1:200~~1000 |
| Storage | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | MAP4K4 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | MAP4K4 (HGNC:6866) |
|---|---|
| Synonyms | HGK, KIAA0687, NIK |
| Function | Serine/threonine kinase that plays a role in the response to environmental stress and cytokines such as TNF-alpha. Appears to act upstream of the JUN N-terminal pathway (PubMed:9890973). Activator of the Hippo signaling pathway which plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. MAP4Ks act in parallel to and are partially redundant with STK3/MST2 and STK4/MST2 in the phosphorylation and activation of LATS1/2, and establish MAP4Ks as components of the expanded Hippo pathway (PubMed:26437443). Phosphorylates SMAD1 on Thr- 322 (PubMed:21690388). |
| Cellular Location | Cytoplasm. |
| Tissue Location | Widely expressed. Isoform 5 is abundant in the brain. Isoform 4 is predominant in the liver, skeletal muscle and placenta. |
Research Areas
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Application Protocols
Provided below are standard protocols that you may find useful for product applications.
References
1. Mol Cell Biol. 2000 Mar;20(5):1537-45. 2. Curr Biol. 2002 Apr 16;12(8):622-31. 3. J Biol Chem. 2007 Mar 16;282(11):7783-9.
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$ 325.00
Cat# AO1191a
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