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MAP4K4 Antibody

Mouse Monoclonal Antiobody to MAP4K4

     
  • WB - MAP4K4 Antibody AO1188b
    Figure 1: Western blot analysis using MAP4K4 mouse mAb against truncated Trx-MAP4K4 recombinant protein (1), MBP-MAP4K4 (aa300-400) recombinant protein (2) and MAP4K4(aa194-436)-hIgGFc transfected CH0-K1 cell lysate(3).
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession O95819
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 3C7B5
Isotype Mouse IgG1
Calculated MW 142101 Da
Description MAP4K4: mitogen-activated protein kinase kinase kinase kinase 4. The protein encoded by this gene is a member of the serine/threonine protein kinase family. This kinase has been shown to specifically activate MAPK8/JNK. The activation of MAPK8 by this kinase is found to be inhibited by the dominant-negative mutants of MAP3K7/TAK1, MAP2K4/MKK4, and MAP2K7/MKK7, which suggests that this kinase may function through the MAP3K7-MAP2K4-MAP2K7 kinase cascade, and mediate the TNF-alpha signaling pathway. Alternatively spliced transcript variants encoding different isoforms have been identified.
Immunogen Purified recombinant fragment of MAP4K4(aa400-500) expressed in E. Coli.
Additional Information
Gene ID 9448
Other Names Mitogen-activated protein kinase kinase kinase kinase 4, HPK/GCK-like kinase HGK, MAPK/ERK kinase kinase kinase 4, MEK kinase kinase 4, MEKKK 4, Nck-interacting kinase, MAP4K4, HGK, KIAA0687, NIK
Target/Specificity Purified recombinant fragment of MAP4K4(aa400-500) expressed in E. Coli.
Dilution WB~~1:500~~2000
Format Ascitic fluid containing 0.03% sodium azide.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsMAP4K4 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name MAP4K4 (HGNC:6866)
Synonyms HGK, KIAA0687, NIK
Function Serine/threonine kinase that plays a role in the response to environmental stress and cytokines such as TNF-alpha. Appears to act upstream of the JUN N-terminal pathway (PubMed:9890973). Activator of the Hippo signaling pathway which plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. MAP4Ks act in parallel to and are partially redundant with STK3/MST2 and STK4/MST2 in the phosphorylation and activation of LATS1/2, and establish MAP4Ks as components of the expanded Hippo pathway (PubMed:26437443). Phosphorylates SMAD1 on Thr- 322 (PubMed:21690388).
Cellular Location Cytoplasm.
Tissue Location Widely expressed. Isoform 5 is abundant in the brain. Isoform 4 is predominant in the liver, skeletal muscle and placenta.
Research Areas
Citations (0)
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References

1. Mol Cell Biol. 2000 Mar;20(5):1537-45.

2. Curr Biol. 2002 Apr 16;12(8):622-31.

3. J Biol Chem. 2007 Mar 16;282(11):7783-9.

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$ 385.00
Cat# AO1188b
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