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PSA (KLK3) Antibody

Purified Mouse Monoclonal Antibody

     
  • WB - PSA (KLK3) Antibody AO1144a
    Figure 1: Western blot analysis using KLK3 mouse mAb against truncated KLK3 recombinant protein.
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  • IHC - PSA (KLK3) Antibody AO1144a
    Figure 2: Immunohistochemical analysis of paraffin-embedded human prostate carcinoma tissues, showing cytoplasmic localization using KLK3 mouse mAb with DAB staining.
    detail
  • IHC - PSA (KLK3) Antibody AO1144a
    Figure 3: Immunohistochemical analysis of paraffin-embedded human prostate tissues using KLK3 mouse mAb with DAB staining.
    detail
  • FC - PSA (KLK3) Antibody AO1144a
    Figure 4: Flow cytometric analysis of PC-3 cells using KLK3 mouse mAb (right) and negative control (left).
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IHC, FC, E
Primary Accession P07288
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 5A11E2
Isotype IgG1
Calculated MW 28741 Da
Description Kallikrein-related peptidase 3.Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. This gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. Its protein product is a protease present in seminal plasma. It is thought to function normally in the liquefaction of seminal coagulum, presumably by hydrolysis of the high molecular mass seminal vesicle protein. Serum level of this protein, called PSA in the clinical setting, is useful in the diagnosis and monitoring of prostatic carcinoma. Alternate splicing of this gene generates several transcript variants encoding different isoforms.
Immunogen Purified recombinant fragment of KLK3 (aa26-251) expressed in E. Coli.
Formulation Ascitic fluid containing 0.03% sodium azide.
Additional Information
Gene ID 354
Other Names Prostate-specific antigen, PSA, 3.4.21.77, Gamma-seminoprotein, Seminin, Kallikrein-3, P-30 antigen, Semenogelase, KLK3, APS
Dilution WB~~1/500 - 1/2000
IHC~~1/500 - 1/2000
FC~~1/200 - 1/400
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPSA (KLK3) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name KLK3
Synonyms APS
Function Hydrolyzes semenogelin-1 thus leading to the liquefaction of the seminal coagulum.
Cellular Location Secreted.
Research Areas
Citations (0)
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References

1. Am J Surg Pathol. 2007 Sep;31(9):1351-5. 2. Chem Biol Drug Des. 2007 Sep;70(3):261-7.

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$ 325.00
Cat# AO1144a
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