CD20 Antibody
Purified Mouse Monoclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| IHC, ICC, E |
---|---|
Primary Accession | P11836 |
Reactivity | Human |
Host | Mouse |
Clonality | Monoclonal |
Clone Names | 3E9D3C1G3 |
Isotype | IgG |
Calculated MW | 33077 Da |
Description | CD20 (MS4A1, membrane-spanning 4-domains, subfamily A, member 1) is a member of the membrane-spanning 4A gene family. Members of this nascent protein family are characterized by common structural features and similar intron/exon splice boundaries and display unique expression patterns among hematopoietic cells and nonlymphoid tissues. The CD20 antigen is present on human pre B lymphocytes and on B lymphocytes at all stages of maturation, except on plasma cells. Low level expression of the CD20 antigen has been detected on normal T lymphocytes. The CD20 molecule is involved in regulation of B cell differentiation, presumably via its reported function as a Ca++ channel subunit. And it is known to accelerate the G0 to G1 progression induced by IGF-1. |
Immunogen | Synthetic peptide corresponding to aa (EPANPSEKNSPSTQY) of human CD20,conjugated to KLH. |
Formulation | Ascitic fluid containing 0.03% sodium azide. |
Gene ID | 931 |
---|---|
Other Names | B-lymphocyte antigen CD20, B-lymphocyte surface antigen B1, Bp35, Leukocyte surface antigen Leu-16, Membrane-spanning 4-domains subfamily A member 1, CD20, MS4A1, CD20 |
Dilution | IHC~~1/200 - 1/1000 IF~~1/200 - 1/1000 WB~~1:500~~2000 |
Storage | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Precautions | CD20 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | MS4A1 |
---|---|
Synonyms | CD20 |
Function | B-lymphocyte-specific membrane protein that plays a role in the regulation of cellular calcium influx necessary for the development, differentiation, and activation of B-lymphocytes (PubMed:12920111, PubMed:3925015, PubMed:7684739). Functions as a store-operated calcium (SOC) channel component promoting calcium influx after activation by the B-cell receptor/BCR (PubMed:12920111, PubMed:18474602, PubMed:7684739). |
Cellular Location | Cell membrane; Multi-pass membrane protein. Cell membrane; Lipid-anchor. Note=Constitutively associated with membrane rafts. |
Tissue Location | Expressed on B-cells. |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
info@abcepta.com, and receive a free "I Love Antibodies" mug.
Provided below are standard protocols that you may find useful for product applications.
References
1. Byar K. Semin Oncol Nurs. 2004,Feb, 20 (1 Suppl 1):20-5. Review. 2. Reiners KS. Hansen HP. Krüssmann A. et al. Immunology. 2004,Jun, 112(2):228-36.
If you have used an Abcepta product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.
If you have any additional inquiries please email technical services at tech@abcepta.com.