Vimentin Antibody
Purified Mouse Monoclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, IHC, E |
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Primary Accession | P08670 |
Reactivity | Human |
Host | Mouse |
Clonality | Monoclonal |
Clone Names | 9E7E7; 5G3F10; 5G3F10E5 |
Isotype | IgG1 |
Calculated MW | 53652 Da |
Description | Vimentin is the major subunit protein of the intermediate filaments of mesenchymal cells. It is believed to be involved with the intracellular transport of proteins between the nucleus and plasma membrane. Vimentin has been implicated to be involved in the rate of steroid synthesis via its role as a storage network for steroidogenic cholesterol containing lipid droplets. Vimentin phosphorylation by a protein kinase causes the breakdown of intermediate filaments and activation of an ATP and myosin light chain dependent contractile event. This results in cytoskeletal changes that facilitate the interaction of the lipid droplets within mitochondria, and subsequent transport of cholesterol to the organelles leading to an increase in steroid synthesis. Immunohistochemical staining for Vimentin is characteristic of sarcomas (of neural, muscle and fibroblast origin) compared to carcinomas which are generally negative. Melanomas, lymphomas and vascular tumors may all stain for Vimentin. Vimentin antibodies are thus of value in the differential diagnosis of undifferentiated neoplasms and malignant tumors. They are generally used with a panel of other antibodies including those recognising cytokeratins, lymphoid markers, S100, desmin and neurofilaments. |
Immunogen | Purified recombinant fragment of Vimentin expressed in E. Coli. |
Formulation | Ascitic fluid containing 0.03% sodium azide. |
Gene ID | 7431 |
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Other Names | Vimentin, VIM |
Dilution | WB~~1/500 - 1/2000 IHC~~1/200 - 1/1000 ICC~~1:200~~1000 IF~~1:200~1000. |
Storage | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Precautions | Vimentin Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | VIM (HGNC:12692) |
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Function | Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally. Plays a role in cell directional movement, orientation, cell sheet organization and Golgi complex polarization at the cell migration front (By similarity). Protects SCRIB from proteasomal degradation and facilitates its localization to intermediate filaments in a cell contact-mediated manner (By similarity). |
Cellular Location | Cytoplasm. Cytoplasm, cytoskeleton. Nucleus matrix {ECO:0000250|UniProtKB:P31000}. Cell membrane {ECO:0000250|UniProtKB:P20152} |
Tissue Location | Highly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone-independent mammary carcinoma cell lines. |
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Provided below are standard protocols that you may find useful for product applications.
References
1. Seshadri, R., et al. Intl. J. Cancer 67: 353-356(1996) 2. Essa, T.M., et al. J. Egyptian Soc. Parasitol. 26:433-442(1996) 3. Chu, Y.W., et al. Amer.J. Pathol. 148: 63-69(1996)
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