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CD3 Antibody
Purified Mouse Monoclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| ICC, E |
|---|---|
| Primary Accession | P20963 |
| Reactivity | Human |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone Names | 4D10A6 |
| Isotype | IgG2a |
| Calculated MW | 18696 Da |
| Description | CD3 is a component of T cell surface receptor (TCR), it cantains 5 invariable chains: gamma- , delta-, epsilon-, and zeta/eta。Clevers et al. (1988) showed the TCR/CD3 complex consists of either a TCR alpha/beta or TCR gamma/delta heterodimer coexpressed at the cell surface with the invariant subunits，CD3 is responsible for the signal transduction of the TCR. |
| Immunogen | Purified recombinant fragment of human CD3 expressed in E. Coli. |
| Formulation | Purified antibody in PBS containing 0.03% sodium azide. |
| Gene ID | 919 |
|---|---|
| Other Names | T-cell surface glycoprotein CD3 zeta chain, T-cell receptor T3 zeta chain, CD247, CD247, CD3Z, T3Z, TCRZ |
| Dilution | IF~~1/200 - 1/1000 WB~~1:500~~2000 |
| Storage | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | CD3 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | CD247 |
|---|---|
| Synonyms | CD3Z, T3Z, TCRZ |
| Function | Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR- mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways (PubMed:2470098, PubMed:1385158, PubMed:1384049, PubMed:7509083). CD3Z ITAMs phosphorylation creates multiple docking sites for the protein kinase ZAP70 leading to ZAP70 phosphorylation and its conversion into a catalytically active enzyme (PubMed:7509083). Plays an important role in intrathymic T-cell differentiation. Additionally, participates in the activity-dependent synapse formation of retinal ganglion cells (RGCs) in both the retina and dorsal lateral geniculate nucleus (dLGN) (By similarity). |
| Cellular Location | Cell membrane {ECO:0000250|UniProtKB:P24161}; Single-pass type I membrane protein |
| Tissue Location | CD3Z is expressed in normal lymphoid tissue and in peripheral blood mononuclear cells (PBMCs) (PubMed:11722641) |
Research Areas
Citations (0)
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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info@abcepta.com, and receive a free "I Love Antibodies" mug.
Application Protocols
Provided below are standard protocols that you may find useful for product applications.
References
1. Clevers, H. Annu. Rev. 1988. Immun. 6: 629-662.
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$ 300.00
Cat# AO1035a
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