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MCL-1 Antibody

Purified Mouse Monoclonal Antibody

     
  • WB - MCL-1 Antibody AO1027a
    Figure 1: Western blot analysis using MCL1 mouse mAb against Hela (1), BCBL-1 (2), Jurkat (3) and HL60 (4) cell lysate.
    detail
  • IHC - MCL-1 Antibody AO1027a
    Figure 2: Immunohistochemical analysis of paraffin-embedded human lymphnode tissues using MCL1 mouse mAb with DAB staining.
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  • IF - MCL-1 Antibody AO1027a
    Figure 3: Confocal immunofluorescence analysis of HepG2 cells using MCL1 mouse mAb (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IHC, ICC, E
Primary Accession Q07820
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 8C6; 8C6D4B1
Isotype IgG1
Calculated MW 37kDa
Description Mcl-1 (Myeloid cell leukemia-1) is Bcl-2-related and was identified as an early-induction gene thatincreased in expression during the differentiation of human myeloblastic leukemia cell ML-1, or exposure to different DNA damaging agents. The level of Mcl-1 is decreased in peripheral B lymphocytes undergoing apoptosis following treatment with apoptotic stimuli such as TGF-alpha 1 and forskolin. Expression of Mcl-1 is able to delay apoptosis induced by over-expression of c-myc in CHO 5AHSmyc cells. In hematopoietic FDC-P1 cells, Mcl-1 interacts with another Bcl-2-related protein, Bax, and prolongs cell viability after treatment with different apoptotic reagents.This monoclonal antibody detected a 37kd MCL1 in BCBL-1 cell lysate.
Immunogen Purified recombinant fragment of human MCL-1 expressed in E. Coli.
Formulation Purified antibody in PBS containing 0.03% sodium azide.
Additional Information
Gene ID 4170
Other Names Induced myeloid leukemia cell differentiation protein Mcl-1, Bcl-2-like protein 3, Bcl2-L-3, Bcl-2-related protein EAT/mcl1, mcl1/EAT, MCL1, BCL2L3
Dilution WB~~1/500 - 1/2000
IHC~~1/200 - 1/1000
IF~~1/200 - 1/1000
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsMCL-1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name MCL1
Synonyms BCL2L3
Function Involved in the regulation of apoptosis versus cell survival, and in the maintenance of viability but not of proliferation. Mediates its effects by interactions with a number of other regulators of apoptosis. Isoform 1 inhibits apoptosis. Isoform 2 promotes apoptosis.
Cellular Location Membrane; Single-pass membrane protein. Cytoplasm. Mitochondrion. Nucleus, nucleoplasm Note=Cytoplasmic, associated with mitochondria
Research Areas
Citations (0)
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References

1. Ota, N. et al. J. Hum. Genet. 2000. 46: 254-269. 2. Schwertfeger KL, Ryder JW, Anderson SM J Mammary Gland Biol Neoplasia 2000, 3 : 236-251.

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$ 300.00
Cat# AO1027a
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